OPTIMUM POTASSIUM CONCENTRATION OF THE PULMONARY PRESERVATION SOLUTION FOR CULTURED PULMONARY ENDOTHELIAL AND SMOOTH MUSCLE CELLS

肺保存液的最佳钾浓度尚未得到系统的研究。为探讨不同钾浓度对培养的肺动脉内皮细胞和平滑肌细胞的影响，分别用低钾低钾溶液(含4 mmoI/L K+)、中钾低钾溶液(含60mmoI/L K+)和高钾低钾溶液(含100mmoI/L K)保存培养的肺动脉内皮细胞和平滑肌细胞，用四甲基偶氮唑盐比色法检测细胞在10ﾟC时的存活率。培养的肺动脉内皮细胞冷藏24小时后，MTT法的吸光度在LIP、MPD和HPD分别为0.147±0.011、0.125±0.013和0.189±0.039(P<0.05)。低温保存24小时后，培养的肺动脉平滑肌细胞的MTT值分别为0.028×0.003、0.042±0.008和0.053×0.008(PK&t；0.05：HPD与LPD和MPD比较)，说明HPD对肺血管内皮细胞和平滑肌细胞的冷保存效果优于LPD和MPD。

The optimal potassium concentration of lung preservation solution has not been investigated systematically. The purpose of this study was to evaluate the effect of solutions with different potassium concentration on cultured pulmonary artery endothelial and smooth muscle cells.A low potassium dextran solution with 4 mmol/L K+(LPD), a moderate potassium dextran solution with 60 mmoI/L K+(MPD) and a high potassium dextran solution with 100 mmol/L K (HPD) were used for preservation.The viability of those cells stored in each solution at 10ﾟC was determined by MTT assay. MTT assay absorbance values for cultured pulmonary artery endothelial cells after 24 hours of cold storage were 0.147 * 0.011(mean*standard error) in LIP, 0.125*0.013 in MPD, and 0.189 * 0.039 in HPD (P<0.05 : HPD vs LPD and MPD). MTT assay absorbance values f* cultured pulmonary artery smooth muscle cells after 24 hours of cold storage were 0.028 * 0.003 in LPD, 0.042*0.008 in MPD, and 0.053*0.008 in HPD (PK<O.05 : HPD vs LPD and MPD).From our results , we conclude that HPD is superior to LPD or MPD in 24-hour cold preservation of pulmonary artery endothelial and smooth muscle cells.