Detection of genetic aberrations in early-stage lung cancer by fluorescence in situ hybridization

应用荧光原位杂交技术（FISH）检测肺癌细胞中p53和erbB2基因位点的遗传畸变。在5/28中检测到p53位点缺失（17.6%），在8/28中检测到erbB2位点扩增（28.6%）。即使在I期病例中也可检测到这两种畸变。我们得出结论，无区域淋巴结转移的外周型小体积肺癌通常在早期发生遗传改变。这些突变的积累可能导致肿瘤的恶性表型。FISH分析的优点之一是术前通过穿刺活检获得肺癌的遗传信息。FISH可能有助于根据遗传畸变的等级选择手术方法。

Genetic aberrations in lung cancer cells were examined using fluorescence in situ hybridization (FISH) concerning the p53 and erbB2 gene loci. Deletions of the p53 locus were detected in 5/28(17.6%) and amplifications of the erbB2 locus were detected in 8/28(28.6%). Both aberrations were detectable even in stage I cases. We concluded that peripheral type small size lung cancers without regional lymph node metastases often carried genetic changes as early events. Accumulation of those mutations may develop malignant phenotype of the tumor. One of the advantages of FISH analyes is to get genetic information of lung cancers by needle biopsy preoperatively. FISH may contribute to select operation methods according to the grade of genetic aberrations.