Detection of chromosomal instability (CIN) in lung cancer cells using FISH

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Chromosomal instability (GIN) in non-small cell lung cancer (NSCLC) has yet to be well studied. We examined the relationship between GIN detected by fluorescence in situ hybridization (FISH) and survival in patients with NSCLC. Touch preparations from 50 surgical specimens of NSCLC were studied. Tumors included 34 adenocarcinomae, 1 5 squamous cell carcinomas, and 1 large cell carcinoma. The pathologic stage was lA in 14, IB in 17, IIB in 8, IIIAin 9, and IIIB in 2 cases. Enumeration ofchromosomes 3, 10, ll, and 17 was used to determine which tumors carried GIN. The association between GIN and survival also was analyzed. Disomy was most common, but tetrasomy anp trisomy of the examined chromosomes were seen frequently. Fourteen umors (28%) showed heterogeneity of all four chromosomes examined and were judged to be carrying GIN. Both univariate and multivariate analyses revealed that two factors, lymph node metastasis and GIN, were significant poor prognostic factors.GIN in NSCLC detected by FISH is an independent factor predicting a poor prognosis.