Detection of chromosomal instability (CIN) in lung cancer cells using FISH

使用 FISH 检测肺癌细胞中的染色体不稳定性 (CIN)

• 批准号: 11671341
• 负责人: NAKAMURA Haruhiko
• 金额: $ 2.37万
• 依托单位: Tokyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671341/
• 关键词: lung cancer; FISH; Chromosome; chromosomal instability; p53; c-erbB2

Chromosomal instability (GIN) in non-small cell lung cancer (NSCLC) has yet to be well studied. We examined the relationship between GIN detected by fluorescence in situ hybridization (FISH) and survival in patients with NSCLC. Touch preparations from 50 surgical specimens of NSCLC were studied. Tumors included 34 adenocarcinomae, 1 5 squamous cell carcinomas, and 1 large cell carcinoma. The pathologic stage was lA in 14, IB in 17, IIB in 8, IIIAin 9, and IIIB in 2 cases. Enumeration ofchromosomes 3, 10, ll, and 17 was used to determine which tumors carried GIN. The association between GIN and survival also was analyzed. Disomy was most common, but tetrasomy anp trisomy of the examined chromosomes were seen frequently. Fourteen umors (28%) showed heterogeneity of all four chromosomes examined and were judged to be carrying GIN. Both univariate and multivariate analyses revealed that two factors, lymph node metastasis and GIN, were significant poor prognostic factors.GIN in NSCLC detected by FISH is an independent factor predicting a poor prognosis.

非小细胞肺癌（NSCLC）的染色体不稳定性（GIN）尚未得到很好的研究。我们研究了荧光原位杂交（FISH）检测的GIN与非小细胞肺癌患者生存之间的关系。研究了50例非小细胞肺癌手术标本的触觉准备。其中腺癌34例，鳞状细胞癌15例，大细胞癌1例。病理分期lA 14例，IB 17例，IIB 8例，IIIB 9例，IIIB 2例。通过对染色体3、10、11和17的计数来确定哪些肿瘤携带GIN。还分析了GIN与生存率之间的关系。二体是最常见的，但检查染色体的四体和三体是常见的。14例（28%）肿瘤显示所有4条染色体的异质性，并被判断为携带GIN。单因素和多因素分析均显示淋巴结转移和GIN是显著的预后不良因素。FISH检测NSCLC的GIN是预测预后不良的独立因素。

项目成果

NAKAMURA H: "Correlation between ended protein overexpression and copy number of the HER2 gene with survival in non-small cell lung cancer"Int J Cancer. 103. 61-66 (2003)

NAKAMURA H：“终止蛋白过度表达和 HER2 基因拷贝数与非小细胞肺癌存活率之间的相关性”Int J Cancer。

Nakamura H: "Correlation between encoded protein overexpression and copy number of the HER2 gene with survival in NSCLC"International Journal of Cancer. 103. 61-66 (2003)

Nakamura H：“编码蛋白过度表达和 HER2 基因拷贝数与 NSCLC 生存的相关性”国际癌症杂志。

中村治彦: "CGH法による肺癌細胞のゲノム変異の解析"日本癌病態治療研究会誌. 5. 178-179 (1999)

Haruhiko Nakamura：“使用 CGH 方法分析肺癌细胞的基因组突变”日本癌症病理学和治疗学会杂志 5. 178-179 (1999)。

NAKAMURA H: "Chromosomal instability detected by fluorescence in situ hybridization in surgical specimens of non-small cell lung cander is associated with poor survival"Clin Cancer Res. (in press).

NAKAMURA H：“通过荧光原位杂交在非小细胞肺癌手术标本中检测到的染色体不稳定性与较差的生存率相关”Clin Cancer Res。

Nakamura H: "Correlation between encoded protein overexpression and copy number of the HER2 gene with survival in NSCLC"Int J Cancer. 103. 61-66 (2003)

Nakamura H：“编码蛋白过度表达和 HER2 基因拷贝数与 NSCLC 生存率之间的相关性”Int J Cancer。