Analyses of molecular mechanism for the host specificity determination in nucleopolyhedroviruses

从斜纹夜蛾（Spodoptera litura，SpltMNPV）和斜纹夜蛾（S.甜菜夜蛾核型多角体病毒（SeMNPV）。这些NPV分离物与来自苜蓿银纹夜蛾（AcMNPV）、桑尺蠖（BmNPV）、美国白蛾（HycuNPV）、假杉毒蛾（OpMNPV）和舞毒蛾（LdMNPV）的NPV一起在来自S. frugiperda（Sf 9）、草地螟S. litura（SpLi）、斜纹夜蛾S. littoralis（CLS-79）、S.甜菜夜蛾（Se 301）、舞毒蛾（Ld 652 Y）、奇异星天牛（Splm）和甜菜夜蛾（B.根据病毒DNA复制、病毒结构蛋白合成、出芽病毒体产量、多角体蛋白合成和多角体形成的数据，发现这些NPV分离物中的每一个与各自的细胞系建立了特异性相互作用。这些相互作用包括不同类型的流产感染和细胞凋亡以及生产性感染。为了深入了解NPV宿主特异性决定的分子机制，鉴定和表征了许多病毒基因，包括gp 64、ie-1（立即早期基因-1）、vlf-1（极晚期因子-1）、iaps（凋亡抑制剂）、p35、hrs（同源重复区）、PCNA（增殖细胞核抗原）和egt（蜕皮激素UDP-葡萄糖基转移酶）。功能分析表明，这些基因中的一些与NPVs感染细胞后的感染性流产和细胞凋亡有关。

A number of clonal isolates of nucleopolyhedrovirus (NPV) were derived from uncloned population from the larvae of Spodoptera litura (SpltMNPV) and S. exigua (SeMNPV) collected in the field. These NPV isolates together with NPVs from Autographa californica (AcMNPV), Bombyx mori (BmNPV), Hyphantria cunea (HycuNPV), Orgyia pseudotsugata (OpMNPV), and Lymantria dispar (LdMNPV) were characterized for their biological properties in seven different cell lines derived from S. frugiperda (Sf9), S. litura (SpLi), S. littoralis (CLS-79), S. exigua (Se301), L dispar (Ld652Y), Spilosoma imparilis (Splm), and B. mori (BmN-4), On the basis of the data oh viral DNA replication, viral structural protein synthesis, budded virion yield, polyhedrin synthesis, and polyhedral formation, it was found that each of these NPV isolates established specific interactions with the respective cell lines. These interactions included varying types of abortive infection and apoptosis as well as productive infection. To provide insights into the molecular mechanisms underlying the host specificity determination of NPVs, a number viral genes including gp64, ie-1 (immediate early gene-1), vlf-1(very late factor-1), iaps (inhibitors of apoptosis), p35, hrs (homologous repeated regions), pcna (proliferating cell nuclear antigen), and egt (ecdysone UDP-glucosyltransterase) were identified and characterized. Functional analyzes suggested that some of these viral genes were relevant to the infectious abortion and apoptosis in the cells infected with NPVs.