Studies on signal transduction in generation and development of cancers.

在vivo中，您的位置：知道173> kinase is the enzyme that produces PIP D23> D2。PIP 3イイD2aイエD2acts as an important second messenger to activate various downstream factors。However，尽管有一个数量级的实验室的影响，但下游因素还没有足够的被识别出来，以解释细胞反应的多样性取决于PI 3-激酶通路。We have produced PIP_D23__既然它与Arf-Gap是同源的，那么很可能这种蛋白质会在载体传输中加入。今年，我们已经建立了一种杂交瘤系列，它是一种用于PIP 3B的绑定蛋白。We also identified SWAP70 as a PIP D23型D2 binding protein which is suggested to be a subunit of a B-cell specific recombinase complex。SWAP 70在细胞系中本地化，但在用一个稳定的活性PI 3-激酶协同表达时移动到核。这种转移对Wortmannin， PI 3激酶抑制剂很敏感。SWAP 70在Sf 9细胞中被过度表达和净化。对SWAP 70的核转移进行的体外分析是不1的--泰德用于ATP相关标记中的核病毒。It is of interest to test the role of in this PIP D23 D2 System。

Phosphatodylinosito1 3-kinase is the enzyme that produces PIPィイD23ィエD2 in vivo. PIP3ィイD2aィエD2 acts as an important second messenger to activate various downstream factors. However, despite the efforts of a number of laboratories, there are not enough downstream factors identified to explain variety of cell responses dependent of PI 3-kinase pathway. We have produced PIPィイD23ィエD2 analogue beads and used them for identification of PIPィイD23ィエD2 binding proteins, which are the candidates for the downstream factors of PIPィイD23ィエD2 PIP3BP is one of such PIPィイD23ィエD2 binding proteins which localizes in the nucleus. Since it is homologous to Arf-GAP, it is likely that this protein is involved in vesicle transport. This year, we established hybridoma lines which produce BP-kinesin, a binding protein for PIP3B. We also identified SWAP70 as a PIPィイD23ィエD2 binding protein which is suggested to be a subunit of a B-cell specific recombinase complex. SWAP70 localized in the cytoplasm but moved into nucleus when co-expressed with a constitutively active PI 3-kinase. This translocation was sensitive to wortmannin, PI 3-kinase inhibitor. SWAP70 was overexpressed in Sf9 cells and purified. In vitro analysis for nuclear translocation revealed that SWAP70 is impo1-ted to the nucleus in an ATP dependent manner. It is of interest to test the role of in this PIPィイD23ィエD2 System.