Study on Mechanisms of Angiogenesis Induced by Blood Flow using in vivo Experimental Model

在雄性Wistar大鼠的大腿上建立微血管动静脉（A-V）分流术以改变血流。人工皮肤真皮（ASD）包裹的A-V分流管周围形成肉芽组织。采用组织学检查、显微血管造影、立体变焦显微镜三维观察血管铸型等方法观察血管结构。在高血流量组，由血流量和血管直径计算的切应力显著增加。扫描电镜观察发现，动脉和静脉的血管腔内都有许多孔洞，提示新血管从袢中长出。动静脉分流环周围新生血管体积逐日增加。新血管的总体积是对照组的四倍。

The microvascular arteriovenous (A-V) shunt was made on the thigh of the male Wistar rats to modify blood flow. Granulation tissues were formed surrounding the A-V shunt wrapped with artifical skin dermis (ASD). A similar control dissection of these vessels was done but no shunt created.The vascular structure was observed by the following methods ; (1) Histologic examinations (2) Microangiography (3) Three dimentional observation of vascular casts with a stereo zooming microscope. In addition, the total vascular volume was calculated from measuring weight of vascular casts.In high blood flow group, shear stress calculated from blood flow and vessel diameter was significantly increased. Scanning electron microscopic observation showed many holes in vessel lumen in both artery and vein, suggesting that new vessels sprout from the loop. There was an increase in the volume of the neovascular around the A-V shunt loop day by day. Total volume of new vessels was four times as great as control.