The discovery of new physiological effect of histamine by gene-manipulated mice and its application for the creation of pathological model

1)阐明HDC基因敲除小鼠。他们缺乏来自全身的组胺；因此对组胺的研究有一定的参考价值。2)我们发现了一些有趣的表型，包括行为、过敏反应和炎症。3)例如，基因敲除小鼠未出现外渗反应，这可以从即刻型皮肤反应中Evan的蓝色渗漏血管中得到证实。此外，这种皮肤反应的消失被口服补充组胺“拯救”。说明立即型过敏反应后的外渗反应成功是受组胺控制的。4)在全身性过敏反应中，组胺已被证明是非常重要的介质之一。5)小鼠白天和夜间的活动也受组胺控制。6)野生型与敲除型对感染的反应不同。7)小鼠在肿瘤浸润和转移中表现出不同反应的可能性很大。我们计划对培养的癌细胞进行紫外线照射或接种，观察其浸润和转移过程。8)制备了在体内表达高组胺的转基因小鼠。我们要用这些老鼠来研究组胺对全身的影响。

1) We elucidated HDC gene knockout mice. They lack histamine from the whole body ; therefore it is useful for the histamine research.2) We found several interesting phenotypes including behavior, allergic reaction, and inflammation.3) For instance, knockout mice showed no extravasation reaction, confirmed by the Evan's blue leakage from the vessel in the immediate-type skin reaction. Moreover, this disappearance of skin reaction was "rescued" by orally supplemented histamine. This shows that the extravasation reaction succeeded after immediate type allergic reaction is controlled by histamine.4) In the systemic anaphylaxis reaction histamine has been proved to be one of the very important mediators.5) The activity of the mice during the day and night is also controlled with histamine.6) Some response to infection was different between wild type and knockout type.7) It will be high possibility that the mice show different response in tumor infiltration and metastasis. We are planning to irradiate ultraviolet rays or inoculate the cultured cancer cells into the mice and observe the infiltrative and metastatic process.8) We prepared transgenic mice expressing high histamine in the body. We are going to use these mice for the study of histamine effects on the whole body.