Development of New System for Production of Liver Specific Proteins using Cell lines Derived from Human Liver by Radial Flow Type Bioreactor

作为肝移植手术的替代方案，许多研究人员一直在朝着功能齐全的人工肝脏的目标努力。近年来，人工肝支持系统被提倡作为等待肝细胞替代治疗或肝移植治疗的患者的临时治疗；所谓的“桥接”治疗。人们认识到，一个有效的人工肝脏系统需要：1)一个有活力和高功能的肝细胞系，2)一个合适的生物反应器环境和外围控制系统，3)一个有效的体外循环系统。然而，传统的系统存在各种缺陷，包括来自非人类细胞的细胞培养物的不兼容性，细胞增殖不足，由于细胞环境不良而导致细胞功能迅速恶化，以及缺乏系统可扩展性。一种新建立的人工肝脏系统克服了许多这些问题，并在定量和定性上证明了长期维持多种肝脏特异性功能的能力，如蛋白质合成、酶活性和药物代谢。

As an alternative to liver transplantation surgery, numerous researchers have been working toward the goal of a fully functional artificial liver. In recent years, artificial liver support systems have been advocated as interim treatments for patients awaiting hepatocyte replacement therapy or liver transplantation treatment ; so-called "bridging" treatments. It is recognized that art effective artificial liver system requires : 1) a viable and highly functional hepatocyte cell line, 2) a suitable bioreactor environment and peripheral control systems, and 3) incorporation within an effective extracorporeal circulatory system. Conventional systems have, however, suffered from various drawbacks including incompatibility of cell cultures deriverd from non-human cells, insufficient cell proliferation, rapid deterioration of cellular function due to an impoverished cellular environment, and lack of system scalability. A newly established artificial liver system overcomes many of these problems and demonstrates a long-term capacity to maintain multiple liver-specific functions, such as protein synthesis, enzyme activity and drug metablism, both quantitatively and qualitatively.