CHARACTERIZATION OF CELLULOSE-BINDING ABILITY OF GEOTRICHUM SP., M111 CELL AND APPLICATION OF DEHYDRATION FOR DISTILLED

采用微管法对地霉M111细胞的纤维结合能力进行了研究。测定了纤维素粉与M111细胞混合物的聚集高度，最适pH为4~5，最适温度为50℃以下。在20 mM柠檬酸盐缓冲液中，30℃时，M111细胞与纤维素纤维的结合常数为0.3%。表面活性剂十二烷基硫酸钠可抑制聚集体的形成。Driselase和Prostase E处理后，M111细胞与纤维的结合能力消失，提示这种结合能力可能与细胞表面蛋白有关。M111细胞的加入加快了甘薯烧酒蒸馏废渣的脱水速度。用线性粘弹性模型对脱水过程进行了分析，结果表明，纤维的加速作用可能是由于纤维间的间距随着细胞的加入而增大的结果。

Cellulose-binding ability of Geotrichum sp., M111 cell was investigated as the aggregation ability with the microtube method. The optimum pH and temperature were pH 4-5 and below 50℃ with measurement of the aggregation height for a mixture of cellulose powder and M111 cell. The binding constant of M111 cell to cellulose fiber was obtained 0.3% in 20mM citrate buffer, pH5.0 at 30℃. The aggregation was inhibited with surfactants such as sodium dodecylsulfate. The binding ability of M111 cell to cellulose fiber disappeared with the treatment of driselase and pronase E. This suggests the binding ability might be due to the cell surface protein. The dehydration rate of distilled waste of sweet potato shochu was accelerated with the addition of M111 cell. The analysis of dehydration with the linear viscoelastic model suggests the acceleration effect might be due to the space increment between cellulose fiber with the cell addition.