STUDIES ON ORIGIN AND PROPERTIES OF A HODGIN CELL LINE, HPL-Hod-2, WHICH PRODUCE CYTOPLASMIC IgG

何杰金氏病是一种独特的恶性淋巴瘤，其特征是Reed-Sternberg （R-S）细胞和何杰金氏(H)细胞的增殖，其正常对应体尚未被确定，尽管最近的证据表明其起源于淋巴细胞。本项目旨在阐明正常的R-S和H细胞对应物，并进一步分析其生物学特性，以了解何杰金氏病的发病机制。研究了21多株恶性淋巴瘤和白血病患者长期培养的细胞株的免疫学特性>其中，从霍奇金病患者的胸腔积液中建立了淋巴母细胞样细胞株hcl - hd -2 (hd -2) > hd -2细胞具有R-S和H细胞的共同细胞标记CD15、CD25、CD30，其细胞质中含有相当数量的IgG。但是，它们缺乏表面免疫球蛋白表达。分子分析表明，这些IgG由h链和k链组成，其分子大小与普通b细胞的胞质IgG几乎相等。有趣的是，发现Hod-2细胞在其细胞表面表达CD83。CD83被认为是树突细胞最可靠的制造者。在21个人类恶性淋巴瘤/白血病细胞系中，除hd -2细胞外，1个毛状白血病细胞系和1个b细胞恶性淋巴瘤细胞系细胞质中表达CD83较弱。总之；本研究发现b系DC的存在及其可能的原细胞为H和R-S细胞。

Hodgkin's disease is a unique malignant lymphoma characterized by proliferation of Reed-Sternberg (R-S) cells and Hodgkin (H) cells, whose normal counterpart have not yet been identified, although the most recent evidence suggests a lymphoid origin.This project aim to elucidate normal counterpart of R-S and H cells and further to analysis their biological properties to understand pathogenesis of Hodgkin's disease.More than 21 long-term cultured cell lines derived from patients with malignant lymphoma and leukemia were investigated their immunological properties> Among them, HPL-Hod-2 (Hod-2), a lymphoblastoid cell line had been established from pleural effusion of a patient with Hodgkin's disease> Hod-2 cells shared common cell markers of R-S and H cells such as, CD15, CD25, CD30 and possessed pretty amount of IgG in their cytoplasms.But, they lacked surface immunoglobulin expression.Molecular analysis revealed these IgG consisted of H-chains and k-chains with almost equal molecular size to cytoplasmic IgG of ordinary B-cells.Interestingly enough, it was found that Hod-2 cells express CD83 on their cell surface. CD83 is thought to be the most reliable makers of dendritic cells.Among 21 human malignant lymphoma / leukemia cell lines, one hairly leukemia cell one and one B-cell malignant lymphoma cell line express weakly CD83 in their cytoplasms besides Hod-2 cells.In conclusion ; this investigation revealed the presence of B-lineage DC and their possible original cells of H and R-S cells.