Immunoelectron microscopic study on the expression of lymphoid markers in HTLV- producing cells

T淋巴细胞表面的T细胞受体（TCR）/CD 3复合物是识别巨噬细胞呈递的抗原所必需的。通常，此类抗原呈递在主要组织相容性复合物（MHC）分子上。另一方面，据报道，HTLV-Ⅰ和-Ⅱ生产者最初是T细胞，在培养过程中其抗原性发生变化，例如失去一些T细胞抗原或获得HLA-DR和/或CD 25抗原，在本研究中，免疫电镜观察，以澄清TCR/CD 3复合物的表达上的人T淋巴细胞病毒（HTLV）的I型和II型生产细胞的表面。WT-31（TCR α/β）染色仅在PM阳性，而αF1（TCR α）和βF1（TCR β）染色仅在NM和rER阳性 ...更多信息 .另一方面，CD 3与NM/rER和PM两者反应。使用这些抗体，清楚地显示正常新鲜、新鲜和短期培养的HTLV-1载体淋巴细胞具有TCR/CD 3复合物，特别是缺乏TCR α/β复合物，本文报道了卡波西肉瘤相关疱疹病毒（KSHV）/人疱疹病毒8型（HHV-8）的超微结构，尽管已经报道了使用原发性渗出性淋巴瘤（PEL）细胞系KS-1的一些发现，但尚未完全阐明，KS-1没有EB病毒（EBV）共感染。在本研究中，KSHV/HHV-8进行详细的精细结构检查后，在体外用12-O-十四烷酰基-佛波醇-13-乙酸酯（TPA）刺激PEL衍生的细胞系KS-1。而核内病毒颗粒不伴细胞外成熟颗粒。用TPA刺激的KS-1细胞产生许多细胞外成熟颗粒以及核内颗粒，除了有趣的管状网状结构和聚集的管状结构的囊泡。诱导的核内颗粒呈空心、圆环形和致密的核心，外径和内径分别为100- 110 nm和60- 70 nm。细胞外成熟颗粒直径为150- 160 nm，部分颗粒核呈环形，并含有少量大病毒，外径为260 nm。这些结果表明，用TPA处理的KS-1细胞可以产生细胞外成熟颗粒以及核内颗粒，这些颗粒被证明是KSHV/HHV-8。少

The T-cell receptor (TCR)/CD3 complex on the surface or T-lymphcytes is required for recognition of the antigens presented by macrophages. Usually, such antigens are presented on Major Histocompatibility Complex (MHC) molecules. In this recognition system, extracellular domains of both TCR α and β chains are reported to be essential for antigen recognition.On the other hand, it has been reported that the antigenicities of HTLV-I and -II producers, which are initially T-cells, change during cultivation, for example loosing some T-cell antigens or obtaining HLA-DR and/or CD25 antigens, as also reported in cases of high grade T-cell malignancy.In the present study, immunoelectron microscopic observation was performed to clarify TCR/CD3 complex expression on the surface of human T-lymphotropic virus (HTLV) type I- and type II-producing cells. Staining with WT-31 (TCR α/β) was found to be positive only in PM, while staining for αF1 (TCR α) and for βF1 (TCR β) was positive only in NM and rER … More . CD3, on the other hand, reacts with both NM/rER and PM.Using these antibodies, normal fresh, and fresh and short-term cultured HTLV-I carrier lymphocytes were clearly shown to possess TCR/CD3 complexes, lacking TCR α/β complex in particular, suggesting that their recognition of antigens is defcctive.The ultrastructure of Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus-8 (HHV-8) has not yet been fully elucidated, although some findings have been reported using primary effusion lymphoma (PEL) cell lines, KS-1, harboring no Epstein-Barr virus (EBV) coinfection. In the present study, detailed fine structural examination of KSHV/HHV-8 was performed after stimulation of the PEL-derived cell line KS-1 with 12-O-tetradecanoy1-phorbol-13-acetate (TPA) in vitro. While nuclear virus particles associated with no extracellular mature particles. KS-1 cells stimulated with TPA produced many extracellular mature particles as well as intranuclear particles, in addition to interesting tubulo-reticular structures and aggregated tubular structures in vesicles. The induced intranuclear particles were empty, doughnut shaped, and dense cored, with outer, and inner diameters of 100-110nm and 60-70nm, respectively. Dense-cored extracellular mature particles were 150-160nm in diameter, and some contained doughnut-shaped cores, together with a few megaloviruses, 260nm in outer diameter. These findings indicate that KS-1 cells treated with TPA can produce extracellular mature particles as well as intranuclear particles, which were proven to be KSHV/HHV-8. Less