The Biological Functions of the SART-1 Family in Differentiation and Carcinogenicity of Adenocarcinomas

Molecules involved in regulation of cellular proliferation at the G2/M phase have not yet been fully identified.This study investigated the involvement of a recently identified SART-1 I D 2800 I D 2.Protein demonstrated an ability to bind to DNA，And accumulated in the nucleus when the cells were arrested at G2/M phase.(L)The localization of SART-1 protein was suggested to be in nucleus of eukaryote cells including monkey kidney EBV-transformed Cos7cell and human esophageal cancer TE9cell when the fusion gene with GFP was transfected and observed by conforcal laser microscopy.(2)SART-1 i D2800i D2 protein is sugested to be DNA binding and observed by conforcal microscopy.(2)SART-1-D2800i D2protein is sugested to be DNA-binding of the fected in the fected of the fected.-1 gene induced the G2/M-arrest and suppressed the cell growth of all the cell tested.Furthermore，DNA ladder formation was observed in the SART-1 transfected COS-7and human cancer cells.The transfection of SART-1up-regulated the nuclear expression of cyclin B and cdc2，well known components of maturation-promoting factor.(4)Several candidate genes which encode proteins that had interacted with SART-1protein were isolated by two-hybrid system.(5)Three different SART-1probe protein(N-terminal，middle and C-terminal regions)for far-western blot analysis of SART-1bing probe protein(N-terminal，middle and C-terminal regions)The sequence of the gene will submit to GenBank.(7)SART-1gene was suggested to be expressed in early development of mouse embryo(L4days)by northern blot analysis.(8)Several family genes of SART-1were suggested by northern blot analysis。One of the gene with 3.3 kb consist of approximately l488bp of 5‘-region was identical to SART-1 and encoded a protein with483amino acid.

Molecules involved in regulation of cellular proliferation at the G2/M phase have not yet been fully identified. This study investigated the involvement of a recently identified SART-1ィイD2800ィエD2. Protein demonstrated an ability to bind to DNA, and accumulated in the nucleus when the cells were arrested at G2/M phase.(l) The localization of SART-1 protein was suggested to be in nucleus of eukaryote cells including monkey kidney EBV-transformed Cos 7 cell and human esophageal cancer TE9 cell when the fusion gene with GFP was transfected and observed by conforcal laser microscopy.(2) SART-1ィイD2800ィエD2 protein is suggested to be a DNA-binding protein by the affinity chromatography of cell lysates of the 293T cells transfected with the HAN/SART-1ィイD2800ィエD2 gene.(3) Transfection of SART-1 gene induced the G2/M-arrest and suppressed the cell growth of all the cell tested. Furthermore, DNA ladder formation was observed in the SART-1 transfected COS-7 and human cancer cells. The transfection of SART-1 up-regulated the nuclear expression of cyclin B and cdc2, well known components of maturation-promoting factor.(4) Several candidate genes which encode proteins that had interacted with SART-1 protein were isolated by two-hybrid system.(5) Three different SART-1 probe protein (N-terminal, middle and C-terminal regions) for far-western blot analysis of SART-1 binding protein candidate obtained by two hybrid system were prepared.(6) We found that murine SART-1 genomic DNA suggested to be consist of 20 exsons. The sequence of the gene will submit to GenBank.(7) SART-1 gene was suggested to be expressed in early development of mouse embryo (l4 days) by northern blot analysis.(8) Several family genes of SART-1 were suggested by northern blot analysis. One of the gene with 3.3kb consist of approximately l488 bp of 5'-region was identical to SART-1 and encoded a protein with 483 amino acid.