CD30 expression in CD4+T cell subsets in children with atopic diseases

人们普遍认为，Th1和Th2细胞之间的平衡必须决定生理和病理免疫反应的结果，包括过敏性疾病。本研究表明，在th2主导疾病的特应性皮炎（AD）患者的外周血中，CD30+细胞在CD45RO+CD4+ T细胞中的百分比与疾病严重程度、血清IgE水平、外周血嗜酸性粒细胞计数以及由白细胞介素(IL)-4与干扰素- γ产生的比值决定的th2主导细胞因子模式的趋势密切相关。此外，我们还研究了AD患者循环T细胞中趋化因子受体表达的体内相关性。研究发现，AD患者血液中表达ccr4的记忆性CD4+ T细胞比正常人增加更多，而表达cxcr3的记忆性CD4+ T细胞在AD患者中的出现频率低于正常人。结合细胞内细胞因子染色的刺激研究显示，能够产生Th2细胞因子的细胞，如IL-4、IL-5和IL-13，仅限于记忆CD4+ T细胞中表达ccr4的细胞群。关于Th1细胞因子的产生，产生干扰素的细胞只存在于表达cxcr3的记忆CD4+细胞中。这些结果表明，CXCR3、CCR4和CD30似乎可以作为识别循环Th1和Th2效应群体的有用标记。评估这些分子在循环T细胞中的表达是一种非常有用和简单的评估体内Th1/Th2平衡的方法。

It is widely accepted that the balance between Th1 and Th2 cells must determine the outcome of physiological and pathological immune responses, including allergic diseases. This study demonstrated that in the peripheral blood of patients with atopic dermatitis (AD) as Th2-dominated disorder the percentages of CD30+ cells within CD45RO+CD4+ T cells correlated well with the disease severity, serum IgE levels, peripheral eosinophil counts, and the tendency toward Th2-dominant cytokine pattern as determined by ratio of interleukin (IL)-4 to interferon-gamma production. Furthermore the in vivo relevance of expression of chemokine receptors on circulating T cells was investigated in patients with AD.It was found that CCR4-expressing memory CD4+ T cells in the blood were more increased in AD patients as compared with normals, whereas CXCR3-expressing memory CD4+ T cells were present in a lower frequency in AD than seen in normals. Stimulation studies combined with intracellular cytokine staining revealed that the cells capable of producing Th2 cytokines, such as IL-4, IL-5, and IL-13, were restricted to the CCR4-expressing population within memory CD4+ T cells. Concerning Th1 cytokine production, interferon-gamma producing cells resided exclusively in CXCR3-expressing memory CD4+ cells. These results suggest that CXCR3, CCR4 and CD30 appear to serve as the useful markers for identification of circulating Th1 and Th2 effector populations. Assessing the expression of these molecules in circulating T cells is a very useful, and easy method to evaluate in vivo Th1/Th2 balance.