Development of the research method for bacterial translocation with indigenous bacteria

在本研究项目中，我们开发了土著细菌并命名为JNW 14。最初从Wistar大鼠粪便中分离的JNW 14用杆菌肽标记，新霉素和链霉素耐药标记，利用土著细菌（JNW 14）的方法是有用的细菌易位（BT）的研究利用JNW 14的动物实验证实了梗阻性黄疸中的BT系统，梗阻性黄疸下的大部分肝切除促进了BT向全身器官和肠系膜淋巴结中存活的JNW 14的数量的增加。肺组织培养阳性组的T细胞计数明显高于阴性组，提示BT的主要途径是胸-腹腔系统，梗阻性黄疸和肝切除术后网状内皮系统功能受损，梗阻性黄疸大鼠回肠形态结构异常，肠通透性增加，给药后肠通透性明显降低，肠梗阻大鼠回肠形态结构异常胆汁酸和去氧胆酸不能降低BT，但全胆汁可降低肠通透性，抑制阻塞性黄疸状态下的BT。

In this research project, we developed the indigenous bacteria and named JNW14. JNW14 originally isolated from Wistar rat feces was labeled with bacitracin-, neomycin-and streptomycin-resistant markers.The method using indigenous bacteria (JNW14) was useful to study for bacterial translocation (BT).The animal study using JNW14 identified the BT system in obstructive jaundice.Major liver resection under obstructive jaundice promoted BT to systemic organs and the number of viable JNW14 in the mesenteric lymph nodes in the lung culture positive group was significantly higher than that of negative group, suggesting the major route of BT is lymphatic-thoracic system.The reticuloendothelial system function was impaired in obstructive jaundice and hepatectomy, and it was mainly due to the decrease of the uptake of bacteria in the liver.Morphology of the ileum showed structural abnormalities and increased intestinal permiability in obstructive jaundiced rat.The administration of cholic acid and deoxycholic acid were not decreased BT, but the whole bile decresed intestinal permiability and inhibited BT under obstrustive jaundice state.