Investigation of the method of the treatment for tracheal stenosis using prosthetic trachea and/or transplantation of tracheal homograft

（研究1）材料与方法：研究MHC Class□抗原对兔气管移植物排异反应最弱的时期，以及气管移植物上皮是否具有再生能力。结果：气管移植物冷冻保存8周后MHC□类抗原下降，保存20周后MHC□类抗原消失。16周后，2 / 3的气管移植物上皮未恢复。结论：气管移植物冷冻保存的最佳时间为8 ~ 12周。需要进一步的研究来澄清这一发现。（研究2）材料与方法：观察胸腺瘤不同分期肿瘤细胞的细胞学变化，评价其浸润性。结果:与细胞学的研究I或II胸腺瘤的阶段,这些阶段III或IV的上皮肿瘤细胞更频繁地透露坏死背景(阶段III或IV vs 11.1% - 50.0%阶段I或II, p = 0.006),大型集群的上皮肿瘤细胞(70.0%比36.1%,p = 0.055),标志着核肿大(90.0%比52.7%,p = 0.033),标志着anisokaryosis(100%比52.7%,p = 0.006),标志着核多态性(40.0%比5.5%,p = 0.004), hyperchromasia (50.0% vs 11.4%,结论：ⅰ期或ⅱ期胸腺瘤与ⅲ期或ⅳ期胸腺瘤上皮细胞的细胞学表现有显著差异，胸腺瘤肿瘤细胞的细胞学表现似乎能够区分非侵袭性和侵袭性胸腺瘤。目的：探讨支气管超声（EBUS）在支气管软骨观察中的应用。结论：EBUS对气管软骨的观察和气管管壁的检查是非常有用的。

(Study 1) Materials and Methods : We studied the period that rejection were weakest in tracheal graft of rabbits, which was influenced by MHC Class □ antigen, and that the epithelium of tracheal graft might be able to regenerate.RESULTS : We found that MHC class □ antigen was decreased over 8 weeks after tracheal graft was preserved in cryogen and MHC class □ antigen was disappeared 20 weeks after that. In two of three cases, the epithelium oftracheal graft were not recovered 16 weeks after that. CONCLUSION : 8 weeks to 12 weeks were best periods to preserve tracheal graft in cryogen. Further study was needed to clarify this findings.(Study 2) Materials and Methods : The cytologic findings of the tumor cells corresponding to the stages in thymomas were investigated to assess their infiltrating nature.RESULTS : Compared with cytologic findings of stage I or II thymomas, those of epithelial tumor cells in stage III or IV more frequently revealed necrotic background (50.0 %-stage III or IV vs 11.1 %- stage I or II, p=0.006), large cluster of epithelial tumor cells (70.0 % vs 36.1 %, p=0.055), marked nuclear enlargement (90.0 % vs 52.7 %, p=0.033), marked anisokaryosis (100 % vs 52.7 %, p=0.006), marked nuclear polymorphism (40.0 % vs 5.5 %, p=0.004), hyperchromasia (50.0 % vs 11.4 %, p=0.007) and prominent nucleoli (50.0 % vs 16.6 %, p=0.028)CONCLUSION : Our findings revealed that there was a significant difference in the cytologic findings of epithelial tumor cells between stage I or II thymomas and stage III or IV those, and the cytologic findings of thymoma tumor cells appears to capacitate to distinguish between non invasive and invasive thymomas.(Study 3) OBJECTIVE : We studied the utility of Endobronchial ultrasonography (EBUS) to observe the bronchial cartilage.Conclusion : EBUS was very useful to observe the tracheal cartilage and we were able to examine the tracheal wall.