The study of disturbance on pulmonary endothelial cell during lung allograft rejection

移植肺在急性排斥反应中的组织学变化为探讨同种异体肺移植排斥反应时内皮细胞损伤与特异性免疫反应的关系，我们研究了供体特异性细胞毒活性和移植肺的组织学变化。结果发现，在细胞毒作用增强时，肺泡间隙有渗出，这似乎是由于内皮细胞的过度渗透所致。本文研究了缓激肽对常温保存损伤的犬肺内皮细胞释放前列腺素I_2能力的影响及其与体内移植肺气体交换功能的关系。结果表明，损伤肺的PGI2释放能力明显降低。认为该方法可用于评估内皮细胞的活性。

Histological changes of the grafted lungs during acute lung allograft rejection To investigate the relation between the injury to endothelial cells and specific immunoresponse during lung allograft rejection, we studied for donor specific cytotoxic activity in recipient PBL and histological changes of the grafted lung. It was found that there was exudation, which appeared to be caused by hyperpermiability of endothelial cells, in the alveolar space at the time of increased cytotoxic activity. It is considered that an evaluation of viability in endothelial cells is essential to study for lung injury due to rejection.Changes of Prostaglandin I2 release capacity from dog lungs PGI2 releasing capacity induced by bradykinin from endothelial cells of dog lung which were injured by normothermic preservation, and correlation between the changes in vitro and gas exchanie function of grafted lung in vivo was investigated. It was found that PGI2 releasing capacity in injured lungs showed significant decrease. It is considered that this assay is useful to estimate the viability of endothelial cells.