Is skeltal muscle cell inplantation able to prevent the irreversible dysfunction of ischemic heart?

本研究的目的是通过成年大鼠心肌细胞培养的经验，我们发现普通的培养方法不适合我们的目的，因为培养皿中的心脏细胞与周围的细胞不能同步收缩，因此我们将培养方法从平板培养改为立体培养，在立体培养的条件下，我们将培养皿中的心脏细胞与周围的细胞进行了比较，结果表明，培养皿中的心脏细胞与周围的细胞不能同步收缩，因此我们将培养皿中的心脏细胞与周围的细胞进行了比较，结果表明，培养皿中的心脏细胞与周围的细胞不能同步收缩。三维培养条件下，细胞寿命可达两个月，用连接蛋白抗体在荧光显微镜下观察到大量的缝隙连接。利用这种培养方法，我们研究了原代大鼠心肌细胞与腹主肌成肌细胞（L6细胞）共培养的适宜条件，并最终确定了混合培养的最佳比例和数量。结果，DAPI标记的L6细胞中观察到类似心脏细胞的形态学变化。

The purpose of this study is to find some interactions during the skeltal muscle cell inplantation into hearts.Through the experiences of our adult rat heart cell cultures, we found that usual culture method for heart cells wasn't suitable for our purpose because heart cells on a dish didn't contract synchronously with cells surrounding it. So we have switched culture method to tri-dimentional culture from flat one.Under the tri-dimentional culture condition, cells maintained their life as long as two months and a lot of gap junctions were observed under fluorescent microscopy using conexin antibody. Then we named the cultured cluster as a functioning cluster because it kept an ability to contract synchronously quite well.By this culture method, we have examined the appropriate condition for the co-culture of primary rat heart cells with skeltal muscle myoblasts (L6 cells), then finaly found the best ratio and numbers for the mixed culture. And a result, morphological changes resembled to heart cells were observed in DAPI labeled L6 cells.