STUDY ON ROLE OF ALDEHYDE IN OXIDATIVE REACTION IN RETINA IN DUCED BY OXIDATIVE STRESS

作为研究视网膜氧化反应影响因素的背景研究，采用免疫组织化学方法对Wistar大鼠和皇家外科学院大鼠的谷胱甘肽过氧化物酶（GSH-PO）定位进行了鉴定。研究发现，杆状细胞外段的退化引起内段线粒体功能障碍，导致GSH-PO从内段向退化的杆状细胞外段迁移。在实验方案（计划于1999年）中，通过光照研究RPE细胞中醛的形成，在添加亚油酸或亚油酸过氧化氢的培养RPE细胞中研究细胞活力。发现荧光灯可引起氧介导的RPE细胞损伤。在计划于1998年和1999年进行的实验方案中，研究了铁诱导的脂质过氧化物衍生的醛和荧光素产物（RFP）的形成，发现RFP的形成随着孵育时间的延长而逐渐增加，白蛋白和HNE的存在在孵育早期急剧增加，随着时间的推移而减少。还观察到谷胱甘肽与HNE缀合物的形成。从这些结果中，得到了以下新的发现。脂褐素类荧光素产物的形成与醛类物质（如HNE）的形成有关，脂质过氧化物所涉及的疾病可能与醛类物质的含量有关，其危害性很大。谷胱甘肽可能有助于减少脂质过氧化物产生的醛的形成。

As the backround study to investigate the factors to influence on the oxidative reactions in the retina, the localization of glutathione peroxidase (GSH-PO) in Wistar rats and Royal College of Surgeon rat was identified by the immuno-histochemical method. It was found that the degeneration of rod outer segment causes the dysfunction of the mitochondria in inner segment, resulting in the migration of GSH-PO from the innner segment to degraded rod outer segment.In the experimental protocol (planned for 1999) to investigate on the formation of aldehyde in RPE cells by illumination, the cell viability was investigated in cultured RPE cell with addition of linoleic acid or linoleic acid hydroperoxide. It was found that fluorescent lamp causes the oxygen species mediated cell injuries to RPE cells.In the experimental protocols (planned for 1998 and 1999) to investigate the formation of aldehydes and fluorescein products (RFP) derived from the iron-induced lipid peroxides, it was found that the formation of RFP increased gradually with the incubation time with the presence of albumin and HNE increased dramatically at early time of incubation and decreased after the time lapse. The formation of glutathione conjugates with HNE also was observed.From these results, the new findings as follos were obtained. The formation of lipofuscin like fluorescein products are related to the formation of aldehydes such as HNE and the diseases in which lipid peroxides are implicated may have an etiological relation with amount of aldehydes to be very harmful. Glutathione may be useful in the reduction of aldehydes formation derived from lipid peroxides.