Electron microscopy and immunohjstochemistry on neurotrophic effects of chorda timpani nerve

1.为了阐明鼓索神经的营养功能，进行了单侧去神经。成熟的真菌乳头切除神经后，乳头萎缩，然后发展形成丝状乳头。当不成熟的真菌形成的乳头被神经切除，它最终改变为一个乳头相同的正常丝状乳头。成熟的真菌乳头神经破碎后，乳头退化，然后开始再分化为正常的真菌乳头。而未成熟的乳头经神经夹伤去神经后，乳头转变为类丝状乳头，部分乳头不能再分化为正常的真菌乳头。这些结果表明，真菌的乳头可能是转化丝状乳头的鼓索神经营养因子诱导和维持。出生后舌下腺失神经支配后，腺泡和肌上皮细胞出现异常。根据这一发现， ...更多信息 鼓索神经参与腺泡细胞和肌上皮细胞的分化.对大鼠Weber腺的组织结构和发育进行了研究。成年Weber氏腺由许多末端部分和导管样结构组成。终末部分为管状腺泡状，混合，由粘液腺泡组成，常被浆液性半月形覆盖。新生大鼠的韦伯腺由四种细胞类型组成：浆液细胞、粘液细胞、中间细胞和未分化细胞。未分化细胞在出生时大量存在，出生后3周消失。Srous细胞在出生后1周时数量众多，然后减少，少数保留为半月形。粘液细胞在出生后1周以前很少出现，出生后3周以后逐渐增多并成为主要的细胞类型。中间细胞在生后2周时数量最多，5周时数量减少并消失。这些发现表明大鼠韦伯腺是分析舌咽神经营养功能最有用的腺体。少

1. To elucidate the trophic function fo chorda tympani nerve, unilateral denervation wasperformed. When mature fungi form papillae were neurectomized, the papillae were atrophic, then progressed to forms resembings filiform papillae. When immature fungi form papillae were neurectomized, it eventually changed to a papilla identical to normal filiform papillae. When mature fungi form papillae were nerve-crushed, the papillae degenerated, then began to redifferentiate to normal fungi form papillae. However, if the immature papillae were denervated by nerve crush, the papillae changed to quasi-filiform papillae, and some of them could not redifferentiate to normal fungi form papillae. These findings suggest that fungi form papillae may be transformed filiform papillae induced and maintained by a neurotrophic factor of chorda tympani nerve. When sublingual gland was denervated at perenatal period, unusual acini and myoepithelial cells were observed. Based on this finding, it is supposed tha … More tthe chorda tympani nerve participates in the differentioation of acinar and myoepithelial cells.2. Histoarchitecture and development of rat Weber's glands were examined. Adult Weber's glands were composed of numerous terminal portions and duct-like structures. The terminal portions were tubuloacinar in shape and were mixed, consisting of mucous acini that often capped by serous demilunes. Weber's glands in the newborn rat were composed of four cell types ; serous, mucous, intermediate, and undifferentiated cells. Undifferentiated cells were abundant at birth and disappear by 3 weeks after birth. Srous cells were numerous at 1 postnatal week, then decrease, with a few remaining as demilunes. Mucous cells were rare until 1 week after birth, then increased and became the predominant cell type after 3 postnatal weeks. Intermediate cells were most numerous until 2 weeks after birth, then decreased and disappear by 5 weeks after birth. These findingssuggest that rat Weber's glands are most useful glands to analyze the trophic function of glossopharyngeal nerve. Less