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Three-dimensional structural studies of oral tissues by ion-etching-scanning electron microscopy.

通过离子蚀刻扫描电子显微镜研究口腔组织的三维结构。

基本信息

批准号：
13671930
负责人：
NAGATO Toshikazu
金额：
$ 1.92万
依托单位：
Fukuoka Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

Three-dimensional structures of the capillary network of thready filiform papillae which locate in the posterior portion of the rat lingual dorsm and hard tissues including bone and tooth are studied with the resin section-ion etching-scanning electron microscopy (SEM). Immunohistochemistry by using resin section-ion etching-SEM method was also examined.The three-dimensional reconstructions revealed that capillary network of the thready filiform papillae exhibit a peculiar shape. One ascendign limb arise vertically from the network of small blood vessels, then branches several times in the primary papilla, and forms hairpin-like loops in the secondary papilla. The tips of the hairpin-like loops are sharp, and many of them have a cul-de-sac-like shape.Ion-etching was performed on the LR White resin sections of rat pancreas to evaluate conditions for immuno-scanning electron microscopy. The present method is suitable for detection images at low magnification that can be correlated with light microscopic observations. Middle to high magnifiications are applicable for detail observations with scanning electron microscopy.Bone tissues of rat's fumerus were analyzed structurally by using resin section-ion etching-SEM method. In the area of haversian canal, the structure of lamella is not clear, and arrangement of osteocytes and collagen fibrils are parallel to the bone axis. In the area of inner circumferential lamellae, the structure of lamellae is clear, but as for the arrangement of some collagen fibrils are parallel, some oblique or some crossing as well as osteocytes, so both of them are variable in direction.

用树脂切片-离子蚀刻-扫描电镜（SEM）研究了大鼠舌背后部丝状乳头及骨、牙等硬组织毛细血管网的三维结构。免疫组织化学染色采用树脂切片-离子蚀刻-扫描电镜法，三维重建显示丝状乳头的毛细血管网具有特殊的形态。一个上行分支垂直地从小血管网络中产生，然后在初级乳头中分支几次，并在次级乳头中形成发夹状环。发夹状的环的尖端是尖锐的，其中许多具有死胡同状的形状。在大鼠胰腺的LR白色树脂切片上进行离子蚀刻以评估免疫扫描电子显微镜的条件。本方法适用于可以与光学显微镜观察相关的低放大率下的检测图像。采用树脂切片-离子蚀刻-扫描电镜方法对大鼠股骨骨组织进行了结构分析。哈弗氏管区骨板结构不清，骨细胞和胶原纤维平行于骨轴排列。内周骨板区骨板结构清晰，但骨细胞及胶原纤维排列方向不一，有的平行，有的倾斜，有的交叉。