Production of Free Radical and Nitric Oxide synthase in Radicular Cysts

中性粒细胞和巨噬细胞产生的自由基和活性氧被认为是攻击细菌或其他对人体有害的刺激物。在任何情况下，如果磁场参与减少溶解的氧气和自由基的合成，假设磁场的作用与氧和自由基的行为有关，磁场可能诱导自由基的形成。为了检查在根尖囊肿中是否产生自由基，分析了自由基合酶的产生。在根管治疗手术时，将患者的根尖周组织全部切除。ESR自旋捕集法检测根尖囊肿内产生的羟自由基. ESR自旋捕集法证实根尖囊肿内有一氧化氮生成，一氧化氮可能在根尖囊肿局部合成，直接参与根尖周病变的病理和生物化学过程.然后，我们将细菌暴露于30、60、80和100 mT的磁场不同时间，以评估磁场对单个细菌物种生长的影响，并且根据磁场的强度，与对照相比，发现所有物种的生长都受到抑制。由细菌感染引起的慢性炎症，可以通过自由基的生物活性进行调节和微妙的平衡。这些概念有助于提高根尖周炎的药物治疗水平。

Free radical and reactive oxygen generated from neutrophil and macrophage are thought to attack bacteria or other harmful irritants to the human body. In any event if magnetic fields are involved in decreasing dissolved osygen and to free radical synthesis assuming that the action of the magnetic field is related to the behavior of the oxygen and free radical, magnetic fields may induce free radical formation. To examine if free radical is produced in radicular cysts, free radical synthase production was analyzed. Periapical tissues were removed from patients at the time of endodontics surgery.Results 1. Hydroxyl radical was generated in radicular cysts by ESR-spin trapping method.2. Nitric oxide was generate in radicular cysts by ESR-spin trapping method and nitric oxide should be synthesized locally to be involved directly in the pathology and biochemistry of periapical lesion.3. We then exposed the bacteria to 30, 60, 80 and 100 mT magnetic fields for different times in order to assess the effect magnetic fields on the growth of the individual species of bacteria, and the growth of all of the species was found to be inhibited, compared to the controls, according to the strength of the magnetic filed.The study concluded that periapical lesions, caused by chronic inflammation with bacteria infection, can be regulated and delicately balanced by the biological activities of free radical. These concepts could lead to improve pharmacological therapy of periapical periodontitis.