Effect of Endodontic Treatment on Arachidonic Acid Metabolism in Radicular Cyst

根尖囊肿越大，破骨细胞就会直接吸收根尖囊肿周围的牙槽骨。前列腺素E2是一种刺激破骨细胞的化学介质。我们已经在科学研究补助金c(1991)中报道了根管治疗对根尖囊肿中花生四烯酸(AA)代谢的影响。本研究采用改良和新增的方法，研究根管治疗对根尖囊内AA代谢的影响。在根管治疗前，用新研制的活检机，在局麻下采集根管囊标本：样本A，在根管治疗后一定时间后，以相同的方式获得根管囊标本：样本B。并用~3H-AA标记48h。检测培养细胞在培养液中和培养细胞中的合成情况。结果，我们可以用新研制的活检仪获取根性囊肿标本，并分析培养细胞的AA代谢。然而，我们建立了这种方法，但我们调查了一些案例。随着病例的增多，通过对样本A和样本B的分析比较，根管治疗对根尖囊内AA代谢的影响将更加明确。

The greater the size of a radicular cyst, osteoclasts directly resorb the surrounding alveolar bone of the radicular cyst. Prostaglandin E2 is one of a chemical mediator which stimulates osteoclasts. We already reported in grant-in-aid for scientific research c (1991) how the endodontic treatment effects on arachidonic acid (AA) metabolism in radicular cyst. This time, we investigated with modified and newly added method how the endodontic treatment effects on AA metabolism in radicular cyst.The samples of radicular cysts were obtained under local anesthesia before the endodontic treatment with newly developed biopsy equipments : sample A.Then certain time was gone after the endodontic treatment, the samples of the radicular cysts were obtained in same manner : sample B.Immediately we obtained the samples, primary cell culture was performed for 72 hours. And labeled with ^3H-AA for 48 hours. After that synthesis from cultured cells into medium for 3 hours and in cultured cells were assayd.As results, we could obtained the samples of radicular cysts with newly developed biopsy equipments and analyzed the AA metabolism of the cultured cells. However we established the method, there were a few cases we investigated. With more cases, compare the analysis of the sample A and sample B,the effects of endodontic treatment on AA metabolism in radicular cyst will be more definited.