The anthropologic study for drug metabolic enzyme CYP2C19 polymor-phisom.

通过Sma I酶切*2多态和BamHI酶切*3多态，建立了CYP2C19基因*1、*2、*3的基因分型。用凝胶电泳法测定扩增产物的酶切片段。2.对109名父母和祖父母有家谱的健康志愿者给予足够的知情同意。定位度是指来自日本东北地区的封闭人口，每个家谱成员被给予1分，而不是来自东北地区的被试被给予0分。用家系树定位度总分作为实验对象的取值。3、对109名志愿者的10毫升血样进行校正。采用建立的聚合酶链式反应-限制性片段长度多态性方法，对109名志愿者的白细胞基因组DNA进行了基因分型，其中49人为零定位，47人为6定位，13人为分类。在零定位度的人群中，*1*1和*1*分别有40%和28%的人携带有CYP2C19基因。而在6个等位基因中，*1*1和*1*2的携带率分别为21%和45%。5、研究了已有文献报道的CYP2C19基因的分布。结果表明，中国蒙古族人群中存在两种不同的CYP2C19基因分布类型，即*1*1和*1*2分别占40%和20%，*1*1和*1*2分别有20%和40%来自波利尼西亚蒙古人。在我们目前的结果中，存在两种类型的日本人的CYP2C19基因，它们起源于欧亚蒙古族和波利尼西亚蒙古族。我们的结果为研究日本人的种族起源提供了非常重要的证据。

1, Genotyping for *1, *2. *3 in CYP2C19 polymorphism were establish by Sma I digestion for *2 polymorphism and BamHI digestion for *3 polymorphism. Digestion fragments of PCR products were determined by gel electrophoresis.2, Enough informed consent was give to one-hundred nine healthy volunteers, who research owns family tree at parents and grandparents. Located degree, which means originated from Tohoku district in Japan as a closed population, were given one point for each family tree member, and were given zero point for subjects without originated Tohoku district. Total points of located degree in family tree were used value of experimental subjects.3, Ten milliliter of blood samples were corrected from 109 volunteers. The CYP2C19 genotypings were determined by previous established PCR-RFLP method using of genomic DNA extracted from white blood cell in 109 volunteers.The 49 experimental subjects had zero located degree, the 47 subjects had six located degree and 13 subjects classified the others. The CYP2C19 genotypes were obtained 40% of *1*1 and 28% of *1* in subjects of zero located degree. On the other hand, the CYP2C19 genotype were obtains 21% of *1*1 and 45% of *1*2 in subjects of six located degree. The different distribution of CYP2C19 genotype were observed between the group zero and six located degree.5, The distribution of genotypes for CYP2C19 in previous reports were researched. The results were suggested that there were two type of Mongoloid groups having different CYP2C19 genotypes distribution, which is population having 40% of *1*1 genotype versus 20% of *1*2 genotype originate from Eurasian mongoloids and population having 20% of *1*1 versus 40% of *1*2 genotype originated from Polynesian mongoloids. In the present our results, there is two type of Japanese population in CYP2C19 genotypes which are originated from Eurasia Mongoloids Polynesian Mongoloids. The our results propose very important evidence for study of Japanese race origin.