HIGH VALUE OF HDL IN CHIPMUNK PLASMA IS CAUSED BY LACK OF CETP

研究了花栗鼠血浆高密度脂蛋白水平较高的原因。人体内CETP和/或HTGL缺陷导致HDL水平升高。在花栗鼠中，CETP和HTGL的活性很低，我们无法检测到CETP蛋白的质量。这些结果强烈表明，花栗鼠体内高密度脂蛋白水平高是由于CETP活性低所致。另一方面，据报道，由基因剪接缺陷引起的纯合子CETP缺乏的人类受试者HDL显着升高。我们通过电泳检测出异常模式，用于花栗鼠基因分析。细节正在审查中。服用purobucol 4周的花栗鼠血浆胆固醇、甘油三酯和高密度脂蛋白分别降至44%、78%和48%。在这些动物中，高密度脂蛋白比率下降了3周，并在4周时恢复。VLDL+LDL在第3周升高，第4周降低。高胆固醇饲粮（添加5%）对雄性动物血浆胆固醇和高密度脂蛋白水平没有影响，但TG在8月龄时比高胆固醇饲粮前降低了47%。高胆固醇喂养8个月后，雌性动物血浆胆固醇水平升高至2倍，TG升高至3倍。然而，HDL水平没有改变。我们现在正试图检测花栗鼠的CETP基因。

We studied on the reason why plasma HDL level of chipmunk is very high. The HDL level is increased by the defects of CETP and/or HTGL in human. In chipmunk, CETP and HTGL activity was very low level, and we could not detect on CETP protein mass. These results suggest strongly that high HDL level in chipmunk due to low activity of CETP.On the other hand, it is reported that human subjects with homozygous CETP deficiency caused by a gene splicing defect have markedly elevated HDL.We detect the abnormal pattern by the electrophoresis for gene analysis in chipmunk. Details are now under examined.The plasma Cholesterol, Triglyceride and HDL was decreased, to 44%, 78% and 48%, respectively, in Purobucol-treated chipmunks for 4 weeks. In these animals, HDL ratio was decreased by 3weeks and recovered at 4weeks. VLDL+LDL was elevated by 3weeks and decreased at 4weeks.High cholesterol diet (5% additive) did not affect the plasma cholesterol and HDL level in male animals, but TG was decreased at 8 month to 47% less than before high cholesterol feeding. In female animals, plasma cholesterol level was elevated to twice, and TG was to threefold at 8 month after high cholesterol feeding. However, HDL level was not changed.We are now trying to detect the CETP gene in chipmunk.