The effects of endocrine disrupting chemicals on wildlife

在这项研究中，我们调查了环境污染物的累积水平及其对野生动物或实验动物生殖器官和肝脏激素动态平衡的影响。我们特别关注环境污染物对食鱼野生动物的影响，因为这些物种中积累了大量的亲脂化合物。为了评估CHC暴露对激素的影响，我们测定了从日本北海道沿海水域采集的大海豹和带状海豹鲸脂中氯代烃(CHCs)的水平。在带状密封件中，随着二邻位多氯联苯(PCB170和180)残留量的增加，总T3水平显著下降。我们还测定了白尾海鹰和白尾海鹰胸肌中有机氯的含量。多氯联苯、滴滴涕等有机农药的检出量较高，有可能造成生殖毒性。此外，我们还调查了其他重金属的积累水平，并在高浓度的野生动物中检测到了三丁基锡(TBT)。因此，我们研究了TBT对发育中的实验动物的影响。孕鼠从妊娠第5天(GD5)至出生后第25天(PND)给予玉米油或TBT灌胃。TBT处理可暂时抑制仔鼠下丘脑和小脑的神经胶质细胞分化。

In this study, we investigated the accumulation levels of environmental pollutants and their effects on the hormonal homeostasis in reproductive organs and livers of wild life or experimental animals. Especially, we focused on the effects of environmental pollutants on fish-eating wild life, since numerous lipophilic compounds were accumulated in these species.Chlorinated hydrocarbon (CHCs) levels in the blubber of Larga seals and Ribbon seals collected from the coastal waters of Hokkaido, Japan were determined in order to assess the hormonal effects of CHC exposure. In Ribbon seals, total T3 levels significantly decreased with an increase of di-ortho PCB (PCB170 and 180) residues. We also determined the organochlorine concentrations in the breaset muscle of Steller's sea eagles and white-tail sea eagles. We detected PCB, DDT and other organopesticides at high levels, which was possible to cause reproductive toxicity. Furthermore, we investigated the accumulation levels of other heavy metals and detected tri-butyltin (TBT) in wild life at high concentration. Thus, we investigated the effects of TBT on the developing experimental animals. The pregnant rats were administrated corn oil or TBT from gestation day (GD 5) through postnatal day (PND) 25. The glial cell differentiations in hypothalamus and cerebellum of pups were transiently suppressed by TBT treatment.