Breeding study of new variety groups in the genus Rhododendron based on molecular phylogenic analysis

为开发杜鹃花属新品种群，进行了序列分析和AFLP分析。由于杜鹃花及其近缘种的CPDNA的matK区和nrDNA的ITS区存在，一些物种在杜鹃花属中的分类位置需要重新构建。门齐蝇属的5种均被纳入杜鹃花分支，表明门齐蝇属可能是杜鹃花的一个亚目。AFLP分析可以揭示门齐菌的遗传多样性。多花杜鹃与几种杜鹃的杂交组合产生了幼苗。对这些幼苗进行AFLP分析，以确认杂种性。通过花粉管伸长和胚发育的显微观察，阐明了部分组合困难的原因。

To develop new cultivar groups in the genus Rhododendron, sequence analysis and AFLP analysis were performed. As a result of matK region of CPDNA and ITS region of nrDNA in Rhododendron and its close related species, taxonomic position of some species should be reconstituted in the genus Rhododendron. All five species of the genus Menziesia were included in the clade Rhododendoron, which indicates Menziesia might be a submenus of Rhododendron. AFLP analysis could reveal the genetic diversity of Menziesia.Some combinations for cross-hybridization between M multittora and several species of Rhododendron produced seedlings. These seedlings were analyzed for AFLP analysis to confirm the hybridity. Microscopic observation of elongation of pollen tube and embryo development were performed to clarify the reason of difficulty in some combinations.