Characterization of acylated anthocyanin and anthocyanin p-coumaroyltransferase in Japanese garden, Iris ensata Thunb

对鸢尾花色素苷酰化酶和花色素苷对香豆酰转移酶进行了研究。进行了表征，并得到以下结果.采用高效液相色谱法对130个栽培品种、13个品系和3个野生型的花色苷进行了分析，并将这些植物的花色苷分为16种主要类型。其中，矮牵牛素3RG 5G-malvidin 3RG 5G、malvidin 3RG 5G-peonidin 3RG 5G、peonidin 3RG 5G-cyanidin 3RG 5G和peonidin 3RG-cyanidm 3RG为新的非酰化类型.研究了酰化花色苷型品种和非酰化花色苷型品种的花色褪色情况，以及锦葵色素3RGac 5G、3RG 5G、矮牵牛色素3RGac 5G和3RG 5G在不同pH条件下的离体稳定性和与异牡荆素的共色素作用。酰化花色苷品种的花色稳定性高于非酰化花色苷品种。锦葵色素3RGac 5G、3RG 5G、矮牵牛色素3RGac 5G和3RG 5G除了在最低的pH 3.2外都不稳定，尽管酰化的花色苷比非酰化的花色苷稍稳定.来自酰化花青素苷品种的花蕾的酶提取物催化对香豆酰基部分从对香豆酰基-CoA转移到花青素3RG和3RG 5G。相反，花青素3G根本不作为酰基受体。花青苷对香豆酰转移酶受显性基因控制，以Ac表示。

In this study, acylated anthocyanins and anthocyanin p-coumaroyltransferase of Iris ensata Thunb. were characterized, and the following results were obtained.1. The anthocyanins of 130 cultivars and 13 lines and 3 wild forms were analyzed by HPLC, and these plants were classified into 16 types of major anthocyanins. Among these types, petunidin 3RG5G-malvidin 3RG5G, malvidin 3RG5G-peonidin 3RG5G, peonidin 3RG5G-cyanidin 3RG5G and peonidin 3RG-cyanidm 3RG were obtained new non-acylated types.2. The fading of flower color in acylated anthocyanin type cultivars and non-acylated anthocyanin ones and the in vitro stability of malvidin 3RGac5G, 3RG5G, petunidin 3RGac5G and 3RG5G due to different pH conditions and copigmentation with isovitexin were examined. The acylated anthocyanin cultivars exhibited higher flower color stability than the non-acylated anthocyanin ones. Malvidin 3RGac5G, 3RG5G, petunidin 3RGac5G and 3RG5G were not stable except at the lowest pH 3.2, although the acylated anthocyanins showed slightly more stable than the non-acylated ones.3. Enzyme extracts from flower buds of the acylated anthocyanin cultivar catalyzed in transfer of the p-coumaroyl moiety from p-coumaroyl-CoA to both the anthocyanidins 3RG and 3RG5G. In contrast, anthocyanidin 3G did not serve as acyl acceptors at all. Moreover, anthocyanin p-coumaroyltransferase was controlled by dominant gene, which is symbolized as Ac.