Efficacy of anti-viral interferon to fish viral diseases

养殖鱼类、日本珍珠牡蛎和日本牡蛎中出现了许多新的病毒性疾病，造成了巨大的经济损失。在本研究中，我研究了重组猫干扰素（rFeIFN）对日本珍珠牡蛎免受akoya病毒感染和日本牡蛎免受“病毒性绿衣病”（VGMD）感染的临床疗效。本品对人工感染人工培养akoya病毒的日本珍珠贝有良好的治疗效果。另一方面，rFeIFN对培养的vgmd病毒感染的日本牡蛎的预防和治疗效果较差。此外，我检查了珍珠牡蛎和日本牡蛎的血细胞上的rfeifn受体，因为血细胞具有吞噬病毒和防止病毒感染病变发展的作用。珍珠牡蛎胞质上约56%的粒细胞具有rfeifn受体。因此，在珍珠牡蛎中，rFeIFN受体的粒细胞被rFeIFN增强了吞噬潜能。另一方面，只有7.3%的日本牡蛎的粒细胞具有rfeifn受体，导致效果较差。

Many new viral diseases occur and caused great economic losses in cultured fishes, Japanese pearl oysters and Japanese oysters.In the present study, I examined the clinical efficacy of administration of a recombinant feline interferon (rFeIFN) to protect Japanese pearl oysters from akoya-virus infection and Japanese oysters from 'Viral green mantle disease' (VGMD). The good efficacy resulted in treating Japanese pearl oysters that were artificially infected with cultured akoya-virus. On the other hand, the rFeIFN administration to Japanese oysters that were infected with cultured VGMD-virus resulted in lesser efficacy in prophylaxis and treatment.Moreover, I examined the rFeIFN-receptor on hemocytes of pearl oysters and Japanese oysters because hemocytes work for phagocytizing viruses and preventing the virally-infected lesions from development. Almost 56% of agranulocytes of pearl oysters possessed the rFeIFN-receptor on the cytoplasms. Therefore, in pearl oyster, agranulocytes with the rFeIFN-receptor were enhanced the phagocytic potential by rFeIFN.On the other hand, only 7.3% agranulocytes of Japanese oysters possessed the rFeIFN-receptor, which caused lesser efficacy.