Squamous cell carcinoma of the lung

自1975年以来，冲绳的肺癌发病率一直是日本最高的，鳞状细胞癌(SCC)，特别是高分化型，是冲绳最常见的形式，尽管高分化型SCC在日本大陆相对较少。此外，冲绳的肺鳞癌中有很高比例的人乳头瘤病毒(HPV)呈阳性。在这项研究中，我们报告了最近组织学特征和HPV感染发生率的显著变化。直到1994年，鳞状细胞癌，特别是高分化型，是最常见的肿瘤类型。然而，自1995年以来，这类病例的数量一直在稳步减少，同时腺癌的发病率略有上升。尽管现在和过去的大多数患者都是重度吸烟者，但鳞状细胞癌的发病率，特别是高分化型，继续稳步下降。此外，在1993年，79%的病例中检测到hpv，尤其是分化良好的bu-…。1995年降至68%，1996年为35%，1997年为23%，1998年为24%。直到1994年，鳞癌，特别是高分化型，是最常见的肿瘤类型。然而，自1995年以来，这类病例的数量一直在稳步减少，同时腺癌的发病率略有上升。尽管现在和过去的大多数患者都是重度吸烟者，但鳞状细胞癌的发病率，特别是高分化型，继续稳步下降。1993年，HPV阳性率为79%，高分化型人乳头瘤病毒(HPV)的感染率在1995年降至68%，1996年为35%，1997年为23%，1998年为24%。用磷酸钙法将pML2中的HPV 6型和pBR322中的16型和18型分别转入培养的腺癌DLD-1(中分化腺癌)和PC-14(低分化腺癌)细胞。以新霉素或潮霉素B为选择标记物。获得了49个人乳头瘤病毒(HPV)转基因细胞克隆。HPV基因转染腺癌细胞后，G0-G1细胞数明显增加。将病毒感染SCID小鼠后，细胞出现鳞状化生，免疫组织化学显示高分子角蛋白(Moll‘s No.1 keratin)和包皮蛋白分子表达，RT-PCR法检测到转谷氨酰胺酶I(Trans谷氨酰胺酶I)基因的表达。与HPV6和18转染组比较，HPV16转染组(16D2)鳞状化生最为明显。较少

The incidence of lung cancer in Okinawa has been the highest in Japan since 1975, and squamous cell carcinoma (SCC), especially the well differentiated form, is the most prevalent form in Okinawa, although well differentiated SCC is relatively rare in mainland Japan. Furthermore, a high proportion of SCC of the lung in Okinawa was positive for human papillomavirus (HPV). In this study, we report recent striking changes in histological features and in the incidence of HPV infection.Up until 1994 SCC, especially the well differentiated form, was the most prevalent type of tumour. However, since 1995 the number of such cases has diminished steadily, accompanied by a slight rise in the incidence of adenocarcinoma. Although most present and past patients are heavy smokers, the incidence of SCC, especially the well differentiated form, continues to decrease steadily. Furthermore, in 1993, HPV was detected in 79% of all cases, and was particularly prevalent in the well differentiated form, bu … More t the rate fell to 68% in 1995, 35% in 1996, 23% in 1997, and 24% in 1998.Up until 1994 SCC, especially the well differentiated form, was the most prevalent type of tumour. However, since 1995 the number of such cases has diminished steadily, accompanied by a slight rise in the incidence of adenocarcinoma. Although most present and past patients are heavy smokers, the incidence of SCC, especially the well differentiated form, continues to decrease steadily. Furthermore, in 1993, HPV was detected in 79% of all cases, and was particularly prevalent in the well differentiated form, but the rate fell to 68% in 1995, 35% in 1996, 23% in 1997, and 24% in 1998.Transfection of human papillomavirus (HPV) type 6 in plasmid pML2, and types 16 and 18 in pBR322 into cultured adenocarcinoma DLD-1 (moderately differentiated colonic adenocarcinoma) and PC-14 (poorly differentiated lung adenocarcinoma) cells respectively using the calcium phosphate method was carried out. Neomycin or hygromycin B was used as selectable makers. 49 HPV transfected cell clones were obtained. The effects of HPV transfection into adenocarcinoma cells were examined.Transfection of the virus into the cultured adenocarcinoma cells increased the G0-G1 cell population markedly. The virus transfected cells showed squamous metaplasia when they were injected into SCID mice, expressing the high molecular weight keratin (Moll's No. 1 keratin) and involucrin molecules immunohistochemically, and transglutaminase I mRNA by RT-PCR.The squamous metaplasia was most conspicuous in the HPV 16 transfected DLD-l (16D2) when compared with HPV 6 and 18 transfected cells. Less