Research on role of chemokine in reproductive physiology

最近，我们已经证明，在大鼠中，固定应激引起趋化能神经元通路的激活，导致细胞因子诱导的中性粒细胞趋化剂（CINC）从下丘脑-神经垂体系统释放到外周血中。这一观察结果促使我们研究胰岛素诱导的低血糖对下丘脑-垂体系统CINC神经元通路的影响。胰岛素注射降低血浆葡萄糖水平，随后血浆ACTH水平迅速升高，血清CINC水平升高。注射胰岛素前，在室旁下丘脑核（PVN）中检测不到CINCmRNA的信号。低血糖刺激在PVN的小细胞和大细胞细分中诱导了cnc mRNA的强杂交信号，表明在下丘脑-神经垂体系统中存在低血糖敏感的趋化能神经元通路。为了研究类似反应在人类中发生的可能性，我们研究了剧烈运动是否会引起血清GRO-α的升高。剧烈运动后血清GRO-α水平明显升高。这些数据表明，GRO-α和CINC一样，通过趋化能神经元通路响应外源应激

Recently, we have demonstrated that in rats an immobilization stress evokes the activation of chemokinergic neuronal pathway which results in the release of cytokine induced neutrophil chemoattractant (CINC) into the peripheral blood from the hypothalamo-neurohypophysial system. This observation prompted us to investigate the effect of insulin-induced hypoglycemia on CINC neuronal pathway in the hypothalamo-pituitary system. An insulin injection decreased the plasma glucose level, which followed a prompt rise in plasma ACTH level and an increase in serum CINC level. Before the insulin injection, the signal for CINCmRNA was undetectable in the paraventricular hypothalamic nucleus (PVN). The hypoglycemic challenge induced strong hybridization signals of CINC mRNA in the parvocellular and magnocellular subdivision of the PVN, suggesting the presence of a hypoglycemia-sensitive chemokinergic neuronal pathway in the hypothalamo-neurohypophysial system. To examine the possibility that similar responses occur in human, we investigated whether strenuous exercise evokes a rise in serum GRO-α. There was a significant rise in serum GRO-α level after strenuous exercise. These data suggest that GRO-α responds to exogenous stress as well as CINC does through chemokinergic neuronal pathway