Analysis of Neural Network regarding Oral Dysfunction

我们使用新生大鼠。我们在体外制备了脑干阻滞剂。应用GABA受体拮抗剂荷包牡丹碱和兴奋性氨基酸NMDA诱导三叉神经节律性活动。记录了三叉神经节律性爆发时三叉神经运动神经元的膜电位。串联电阻为60.9±6.8MΩ，全细胞电容为3.5±2.6pF，静息电位为50.7± 6.8mV。除极后，动作电位随EPSP的增加而增强，说明荷包牡丹碱引起膜电位的除极，NMDA在节律的产生中起一定作用。分别从三叉神经、三叉神经运动核和舌下神经进行细胞外记录。在记录呼吸活动后，将荷包牡丹碱和NMDA注入室中以诱导节律性三叉神经活动。三叉神经节律性活动增强后，呼吸活动减弱。在咀嚼和呼吸等运动活动中，分离的脑干阻滞标本对分析神经网络非常有用。我们将继续使用这种制备来分析这些神经回路。

We used the neonatal rats. We made the brainstem block preparation in vitro. We applied GABA receptor antagonist, Bicuculine and the excitatory amino acid, NMDA to induce the rhythmical trigeminal activities. We recorded the membrane potentials of trigeminal motoneuron during rhythmical trigeminal bursting. The series resistant was 60.9±6.8MΩ, whole cell capacitance was 3.5±2.6pF and resting potential was 50.7±6.8mV.After application of Bicuculine and NMDA, firstly EPSP was recorded in the membrane potential. After depolarization, the action potential was enhanced with each EPSP.We concluded that Bicuculine induces depolarization of membrane potentials and that NMDA plays some role in rhythm generation.We also made the brainstem block including respiratory circuit. We made the extracelluler recording from trigeminal nerve, trigeminal motor nucleus and hypoglossal nerve. After recording respiratory activities, we applied Bicuculine and NMDA to the chamber to induce the rhythmical trigeminal activities. After enhancement of the rhythmical trigeminal activities, the respiratory activities decreased. It is likely that there is an antagonistic relationship between these two circuits.In the motor activities such as mastication and respiration, an isolated brain stem block preparation is very useful to analyze the neural network. We will continue the analysis of these neural circuits using this preparation.