ESTIMATION OF FLUORIDE ANALYSIS FOR FOOD BY MICRODIFFUSION METHOD

为了建立个人每日氟化物摄入量(DFIs)的标准，必须进行氟化物分析，以确定全球范围内的氟化物摄入量(AI)。这些数据将监测用于预防龋齿和口腔疾病的氟化物应用的氟化物摄入量。这项研究的目的是估计婴儿配方奶粉、婴儿食品和商业日常食品的食品样本的DFI。氟化物测定采用六甲基二硅氧烷-微扩散法，测定温度60℃，时间12小时以上。含氟0.1ppm的婴幼儿配方奶粉(即食)氟含量范围为0.14~0.22ug/ml(平均0.17ug/ml，h=10)，鸡肉、鱼肉、作为婴儿食品的蔬菜和果汁的氟含量平均值分别为0.30(n=11)、0.13(n=20)、0.17(n=11)和0.10ug/g(n=6)。另一方面，各食物组的平均氟浓度分别为牛奶0.05ug/g，鸡蛋0.08ug/g，…0.023ug鱼和肉，蔬菜0.06微克/克，土豆和淀粉0.02微克/克，水果0.07微克/克，谷类0.14微克/克和糖0.07微克/克。在日本非氟地区，饮料中氟的平均值为0.46微克/克，饮用水中氟的浓度为0.1毫克/升。根据日本食物组成标准表中推荐的食物摄入量进行计算。3-8月龄婴儿平均DFI值为0.166-0.266 mg/d，1-6岁DFI值为0.289-0.321 mg/d。3~8月龄婴儿的DFI值为0.023~0.29 mg/kg，1~6岁的DFI值为0.027~0.011 mg/kg，低于美国国家科学院食物与营养委员会规定的氟斑牙发生的临时性标准0.05、0.07 mg/kg。这些结果表明，在不同饮用水氟浓度的区域指南中，应用氟化物的有效性和安全性应与估算氟的适宜摄入量(AI)有关。较少

To establish the criteria of individual daily fluoride intakes (DFIs), fluoride analysis is necessary to ascertain the adequate intake (AI) of fluoride with available world-wide basis. These data are to have a monitoring of fluoride intake in terms of fluoride application for the prevention of dental caries and oral diseases. The purpose of this study was to estimate DFIs for food samples of infant formula, baby food and daily foods available commercially. Fluoride was determined by hexamethyldisiloxane(HMDS)-microdiffusion method at 60℃ for 12hr over. Fluoride concentrations of infant formula (ready-to feed) with 0.1ppm fluoride drinking water ranged from 0.14 to 0.22ug/ml (mean 0.17ug/ml, h=10), and fluoride contents of coreal, meat and fish, vegetable as baby food and juice showed mean value of 0.30 (n=11), 0.13(n=20), 0.17 (n=11) and 0.10ug/g (n=6), respectively. On the other hand mean fluoride concentration of each food group showed that 0.05ug/g in milks, 0.08ug/g in egg, 0.023ug … More /g in fish and meat, 0.06ug/g in vegetables, 0.02ug/g in potatoes and starches, 0.07ug/g in fruits, 0.14ug/g in cereals and 0.07ug/g in sugars. Fluoride in beverages showed the mean value of 0.46ug/g and fluoriide concentration in drinking water assumed the level of 0.1mg/1 in non fluoridated area in Japan. In calculation based on food intakes of recommendation in standard tables of food composition in Japan. The estimated mean DFIs for infant 3-8months of age were renged from 0.166-0.266mg/day and mean DFIs in 1-6 years olds were showed values of 0.289-0.321mg/day. DFIs por body weight ranged from 0.023-0..29mg/kg in infant of 3-8months of age and from 0.027-0.011mg/kg in 1-6years of age, that wore further lower levels than 0.05, 0.07mg/kg which is temperarily the criteria of the occurrence of onamel fluorosis (Food ＆ Nutrition Board, Institution of Medicine-National Academy of Sciences, USA). These results indicated that effectiveness and safety of fluoride applications should be available with on the estimation of AI (adequate intake) of fluoride in regional guidelines for defferent fluoride concentrations in drinking water. Less