In vitro preservation of medicinal plants

本研究项目成功地建立了日本东葛试管苗的快速繁殖体系和中期保存方法。(茄科)和苍术属(苍术)。(菊科)。试管苗在2℃条件下贮藏2年，效果良好。在新配制的含有吲哚-3-乙酸(1μM)和6-苄基腺嘌呤(10μM)的Linsmaier和Skoog培养基上进行再生，成活率达100%。试管苗在不含植物生长调节剂或吲哚-3-乙酸(1μM)的LS培养基中生根。3、4年生的日本血竭试管苗和大田栽培1-2年的毛刺再生苗在形态和生化特征上均未表现出明显的变异，即毛刺根茎中托烷生物碱的组成和含量以及白术挥发油中的倍半萜成分苍术。结果表明，药用植物试管苗培养的非冷冻冷藏可以作为种质资源的中长期保存系统，而不会对其生化和生物学特性造成劣化。

The present research project was successful in micropropagation protocol and medium term preservation method for in vitro shoot culture of Scopolia japonica Maxim. (Solanaceae) and Atractylodes ovata DC. (Compositae). In vitro grown shoots were successfully stored at 2℃ up to 2 years. Reculturing of the shoots in freshly prepared Linsmaier and Skoog medium supplemented with indole-3-acetic acid (1μM) and 6-benzyladenine (10μM) showed hundred percentage viability. Excised shoots were rooted in LS medium containing none of plant growth regulators or indole-3-acetic acid (1μM).Three- and four-year-old in vitro raised plants of S.japonica and the regenerated plants of A.lancea cultivated in the field for one or two years did not show any detectable variability in their morphological as well as biochemical characters, i.e. the composition and contents of tropane alkaloids in the S.japonica rhizome and the contents of atractylon, a sesquiterpene component of essential oil in the A.lancea rhizome, respectively, when compared with the each control set.Results reveals that non-frozen cold storage of in vitro shoot cultures of medicinal plants can be used as a germplasm preservation system for a medium-duration without deterioration of their biochemical as well as biological characteristics.