Mechanism of dye decoloration using enzyme system bleaching agent for the purpose of environmental loading reduction-Reaction selectivity of dye-

以3种颜料为原料，考察了混合体系对染料的反应。氧化还原电位越小，脱色速率常数越大。虽然氧化还原电位相等，但速率常数不同的原因是染料的空间结构不同。在染料混合体系中，即使橙色II和橙色G共存，橙色I与HRP的反应也不有效，而橙色I的反应迅速进行。与此同时，橙色II减速，橙色G加速。这似乎是由于酶的底物特异性和染料氧化还原电位之间的差异导致自由基染料生成速率不同的原因。因此，预期染料混合体系的反应机理如下。在染料混合体系中，由于反应的橙色I比其他染料的反应速度要快得多，所以自由基是从反应后的右边生成的，然后分解。在此期间，由于Orange II的氧化还原电位较高，Orange II的反应没有开始，而Orange I则先与酶反应。橙色II的反应开始于橙色i的反应结束之后，橙色G的反应开始也较晚。基于以下原因，混合体系对橙II的反应速度也比单一体系慢。在充当橙色G的活化剂时，橙色II自由基接受橙色G中的电子，橙色II自由基返回橙色II。认为在染料混合体系中，橙二的反应比橙二单一体系受到更大的抑制。

Using 3 kinds of pigments, the reaction which used the dye by the mixed system was examined. The decoloration rate constant increases more, as the oxidation-reduction potential is smaller. It is considered that the reason why the rate constant differs is because the dye space structure differs, though oxidation-reduction potential is equal. In the dye mixed system, it was not effective for the reaction between Orange I and HRP, even if Orange II and Orange G coexist, and the reaction of Orange I rapidly progressed. In the meantime, Orange II slowed down, and Orange G quickened. It seems to be a cause that the radical dye generation rate differs on this by the difference between enzymatic substrate specificity and dye oxidation-reduction potential. Therefore, the reaction mechanism of anticipated dye mixed system consists like the following. In case of the dye mixed system, since Orange I of the reaction is very rapider than that of other dye, the radical is formed from the right after reaction, and it decomposes afterwards. In the meantime, the reaction of Orange II does not start, since the Orange II oxidation-reduction potential is high, while Orange I is reacting with the enzyme prior. The reaction of Orange II starts after the reaction end of Orange I.It is also similar that reaction start of Orange G is late. It is based that the reaction rate of Orange II is also slower than the single system by mixed system on following reasons. In doing the role of the activator of Orange G, the orange II radical receives the electron in Orange G.The orange II radical returns in Orange II.It is considered that in the dye mixed system, the reaction of Orange II is suppressed further than the Orange II single system.