Study of Life Science as Complex Systems

我们建立了复杂系统生物学的基础，揭示了所有生命系统的普遍特征，采取建设性的方法，从实验和理论上建立了一个简单的系统。(1)由DNA和DNA聚合酶等组成的体外人工复制系统的合成。理论证明，数量较少的分子开始发挥遗传作用，从某种意义上说，它们相对较强地控制细胞的行为，并被保存得很好。(2)脂质体中蛋白质合成和rna复制的成功，脂质体稳定自我复制的观察和构建，以及脂质体上的dna杂交。(3)理论上发现了维持递归生产的细胞中化学丰度的普遍统计规律，即平均基因表达的幂规律和每种化学物质丰度的对数正态分布，并得到了实验证实。(4)in-Vit…使用激活素和一些其他蛋白质，从异种未分化细胞中获得几乎所有器官的更多再生。跳跃现象与社区效应的发现。证明构建的眼和心脏组织在移植到生物体内后正常工作。(5)基于耦合动力系统的细胞分化和发育过程理论，以显示稳健性和不可逆转性。(6)利用嵌入的基因网络构建E-Coli的分化。(7)E-Coli的进化表明多样性是在拥挤的条件下诱导的。(8)共生共生物种形成理论的提出。(9)E-Coli和阿米巴共生系统的构建。(10)进化速度与克隆的表型波动成正比的理论命题，人工选择实验证实可以增加大肠杆菌中蛋白质的荧光。(11)开发一种设备系统，允许长期观察、操纵单细胞和芯片上的细胞分选器。较少

We have set up the basis of complex systems biology, to unveil universal features underlying all life systems, by taking a constructive approach, to set up a simple system both experimentally and theoretically.(1)Synthesis of in-vitro artificial replication system consisting of DNA and DNA polymerase and others. Theoretical demonstration that molecules minority in number start to play the role of heredity, in the sense that they control the behavior of a cell relatively strongly and are preserved well. This theory is confirmed by the above experiment.(2)Success of protein synthesis and RNA replication in liposome, observation and construction of stable self-replication of liposomes, and DNA hybridization on liposome.(3)Theoretical discovery of universal statistical laws of chemical abundances in a cell that sustains recursive production, i.e., a power law in average gene expression and log-normal distribution of the abundances of each chemicals, as is confirmed experimentally.(4)In-vit … More ro genesis of almost all organs from Xenopous undifferentiated cells, using activin and a few other proteins. Discovery of jump-over phenomena and community effect. Demonstration that the constructed eye and heart tissues work normally after transplantation to organisms.(5)Theory of cell differentiation and developmental process based on a coupled dynamical systems, to show robustness and irreversibility.(6)Construction of differentiation by E Coli by using embedded gene network.(7)Evolution of E Coli to show that diversity is induced in a crowded condition.(8)Proposition of a theory of symbiotic sympatric speciation.(9)Construction of symbiotic system by E Coli and amoeba.(10)Theoretical proposition that evolution speed is proportional to phenotypic fluctuation of clones, as confirmed by artificial selection experiment to increase fluorescence of protein in E Coli.(11)Development of an equipment system that allows for long-term singe cell observation, manipulation, and on-chip cell sorter. Less