Evaluation method development of endocrine disruptor effect and pollution analysis by its application

已经开发了一些类型的装置，用于在免疫反应后电化学测定特定的化学物质。在其中一个装置中，SPR池被用作反应场。通过SPR信号监测抗体的固定和免疫反应，并通过电化学检测酶反应的最终产物。该方法的优点是可以监测夹心免疫分析过程的复杂步骤，并可以成功地持续到最终的电化学酶分析。只有当抗原浓度较高时，SPR信号才能检测到抗原，电化学检测的灵敏度比SPR高1000倍。利用表面等离子体共振技术在金膜上制作了反应场。通常，固定化需要较长的时间，如一个晚上，当抗体与戊二醛连接在玻璃基板上时。相反，抗体可以在短时间内固定在金膜上。使用SPR仪器时，固定化面积有限。但是，通过使用大的固定化面积，可以在没有用于温育的流动停止的情况下进行酶反应。另一个装置由微毛细管组成。该毛细管装置只需5 μL样品即可测定。IgG的检出限很小，为2阿莫尔。采用GC/MS法对生物和沉积物中的PCB含量进行了分析，结果表明，底质中的PCB含量高于湾口。B的许多样品中的多氯联苯水平。测量了大胸鳍鱼。浓度与体长有关，与年龄无关。

Some kinds of devices have been developed to determine specific chemicals electrochemically after immunoreactions. In the one of the devices, a SPR cell was used as a reaction field. Immobilization of antibody and immunoreactions were conducted with monitoring with the SPR signal and final product by enzyme reactions were detected electrochemically. This method had an advantage that the complicated procedure of sandwich immunoassay process could be monitored and could be successfully continued to the final electrochemical enzyme assay. The antigen could be measured by the SPR signal only when the concentration was high, and the electrochemical determination had 1000 time greater sensitivity than the SPR's. A reaction field was fabricated on a gold film by the process establish with the help of the SPR. Usually, the immobilization needs long time such as one night, when the antibody was conuected on a glass substrate with glutalaldehyde. On the contrary, the antibody could be immobilized on the gold film in a short time. When we used SPR instrument, the immobilization area was limited. But, by using the large immobilization area, the enzyme reaction could be done without the flow stop for the incubation. The other device consisted of a micro-capillary. With this capillary device, only 5 μL of sample was needed for the determination. The detection limit of IgG was very small, 2 amol. This device was expected to be used with inserting into a living body directly.PCBs contained in living things and sediments were investigated by means of GC/MS. It was found that the PCB level at the bottom was higher than the mouth of the bay. PCBs levels in many samples of B. pectinirostris were measured. The concentration was related to the body length rather than to their age.