Evaluation for stock efficiency of fish and shellfish in Hiroshima Bay by DMA marker

本研究分离了4个高度变异的微卫星标记，并利用它们研究了采自日本西部和韩国南部6个地点的黑鲷野生种群和采自广岛湾的黑鲷孵化场种群的遗传多样性和种群结构。日本西部和孵化场种群之间的遗传多样性没有差异。根据Nei氏遗传距离，没有地理分离的群体在日本西部地区的群体，我们调查了遗传变异和系谱追踪的51个育种家生产的7个微卫星座位，2000年和2001年。虽然从亲鱼到后代的平均杂合性没有变化，但每个位点的平均等位基因数减少了16.5%以上。对于69.3%（2000年为69.7%，2001年为69.0%）的后代，一个父母对确定使用系谱分析。2000年的实际缴费率为女性51.7%，男性77.3%，2001年为女性48.3%，男性72.7%。女性和男性的贡献率分别为69.0%和90.9%，这表明超过30%的女性没有参加任何一年。使用实际参与贡献者的数量并校正每个父母的不平衡家庭规模，贡献者的有效数量（Ne*）为40。当释放后用7个微卫星位点进行股票分离研究时，分离效率与ALC标记在耳石中几乎相同。这些微卫星位点具有很强的遗传标记的可能性，黑鲷的遗传变异和分化。我们在海参和岩鱼中分离了微卫星作为DNA标记。

Four highly variable microsatellite were isolated and then used to investigate genetic diversity and population structure in black sea bream, Acanthopagrus schlegeli, collected from wild population of six locations (western Japan and southern Korea), and hatchery stock from the Hiroshima Bay. There were no differences in genetic diversity between western Japan and hatchery-stock populations. According to the Nei's genetic distance, there was no geographically separated population in western Japan populations.We surveyed the genetic variability and pedigree tracing of stocked black sea bream produced from 51 breeders with 7 microsatellite loci for two years, 2000 and 2001. While the mean observed heterozygosity was not changed from broodstock to offspring, the mean number of alleles per locus decreased more than 16.5%. For 69.3% (69.7% in 2000 and 69.0% in 2001) of the offspring, one parental couple was determined using pedigree analysis. The actual contribution rate was 51.7% of females and 77.3% of males in 2000 and 48.3% of females and 72.7% of males in 2001. The evidence that contribution rate of female and male was 69.0% and 90.9% in totaling two years, respectively suggests more than 30% of females did not participate in either year. Using the number of actually participating contributor and correcting for unbalanced family size per parent, the effective number of contributors (Ne*) was40. When stock separation study conducted using 7 microsatellite loci after release, separation eddiciency was almost identical to ALC-tagging in Otolith. The microsatellite loci showed strong possibilities as genetic markers of black sea bream for genetic variability and divergence. We isolated microsatellite as a DNA marker in sea cucumber and rock fish.