Study of a naphthalene diimide derivative which can amplify the information of DNA duplex formation

开发高灵敏度、快速的基因检测系统对基因诊断和基因治疗都具有重要意义。本项目的目的是对基于线型DNA插入物衍生物的高灵敏度和快速基因检测系统的开发进行基础研究，该系统有望具有高边际的单链DNA （DNA探针）和双链DNA（与目标DNA杂交）的识别能力。具体而言，我们合成了一种新型的螺纹型插入剂，即在两个亚胺取代基末端携带生物素片段的萘二亚胺，并通过分光光度法和流体动力学方法研究了它们与单链和双链DNA的相互作用。此外，将该插层器应用于亲和素碱性磷酸酶产生的不溶性染料偶联的膜检测系统。虽然这种插入器没有表现出这样的特性，但这里得到的结果对将来设计更好的基因检测系统是有用的。

Development of a highly sensitive and rapid gene detecting system is becoming important with respect to both gene diagnosis and gene therapy. The aim of this project is the basic study of the development of a highly sensitive and rapid gene detecting system based on threading-type DNA intercalator derivatives which are expected to possess a discrimination ability between single (DNA probe) and double (hybrid with target DNA) stranded DNA with high margin. Specifically, we synthesized a new threading-type intercalator, naphthalene diimide carrying biotin moieties at the termini of both imide substituents, and studied their interaction with single and double stranded DNA by means of spectrophotometric and hydrodynamic methods. Additionally, this intercalator was applied to the detection system of the membrane coupled with an avidin-alkaline phosphatase-generating insolubledye. Although this intercalator did not show such a property, the result obtained here is useful for the design of a better gene detection system in the future.