Studies on diagnosis and pathogenicity of Pasteurella pneumotropica in mice.

作者着重研究了肺热带巴氏杆菌的输送介质和细菌形态。莱博维茨介质的能力。用0.5%琼脂（L-15运输培养基）、磷酸盐培养基（0.3%磷酸二钠）和Cary-Blair培养基对3株肺炎假单胞菌的存活率进行了研究。L-15运输培养基优于其他培养基，使生物体在室温下存活7天以上，而不减少活菌的数量。用透射电镜研究了小鼠和大鼠肺炎假单胞菌在加或不加多阳离子铁蛋白标记的情况下细胞表面的结构。小鼠分离株、大鼠分离株和小鼠对照株ATCC 35149均具有革兰氏阴性菌的细胞表面特征。小鼠和大鼠品系之间存在差异。大鼠菌株细菌细胞死亡较多，小鼠菌株细菌细胞存活较好。此外，还研究了菌株的生物学和生化特性。所有菌株均鉴定为Jawetz型。

The author focused this studies on transport medium for pasteurella pneumotropica and morphology of the bacteria. The ability of leibovitz medium no. 15 with 0.5% agar (L-15 transport medium), phosphate medium (0.3% disodium phosphate ), and Cary-Blair medium to preserve the viabilities of 3 P.pneumonia strains was studied. L-15 transport medium was found to be superior to the other media, enabling survival of organism for more 7 days at room temperature without decreasing the numbers of viable P.pneumotropica. The structures of cell surfaces with the strains of P.pneumonia from mouse and rat were studied by transmission electron microscopy with or without polycationic ferritin labeling. The 3 strains of P.pneumotropica, isolates of mouse, rat, and reference strain from mouse, ATCC 35149 , have characteristic cell surfaces of gram negative bacteria. There are differences between mouse and rat strains. Many bacteria cells of rat strain were dead, whereas those of mouse strains were good survived. Furthermore, biological and biochemical properties of the strains were investigated. All strains were identified as type Jawetz.