Study of functional model for metal containing hydrases by new tripot metal complexes

合成了Cu(II)TACH和Zn(II)TACH （TACH=顺式，顺式，1,3,5-三氨基环己烷），并通过x射线结构分析证实了它们的应变结构。以Cu(II)TACH为原料，在pH 7 ~ 10、70℃条件下对几种二肽进行了水解和形态形成研究。用ZnCl_2、ZnHEN、ZnCD-AMP对几种脱肽类水解进行了加速效果评价。测定了游离肽（GlyGly、GlySer、SerGly、LeuGly、GlyLeu、SerMet、AlaSer、LeuSer、ValSer、GlyThr、AlaThr、GlyGlyGly）在pH 7 ~ 10、70℃下的动力学和热力学数据。在pH 7 ~ 9.5、35℃的条件下，用Cu(II)TACH-X（X=H, Me, Et, 2Et, 3Et）水解pBR322 DNA，研究反应机理和X的作用。用Cu(II)TACH裂解环DNA(pHSG398, pUC19, pBR322, φX174, ColE1)，研究DNA尺寸效应。同时，建立了一种测定DNA断裂位点的新方法。

Cu(II)TACH and Zn(II)TACH (TACH=cis, cis,1,3,5-triaminocyclohexane) were synthesized and their strained structures were confirmed by X-ray structure analysis. Hydrolysis and speciation study of several dipeptides with Cu(II)TACH was performed at pH 7-10, 70℃ to estimate the mechanism and the reaction intermediate. Hydrolysis of several depeptides with ZnCl_2, ZnHEN, ZnCD-AMP were followed to evaluate the acceleration effect. Also kinetic and thermodynamic data of free peptides (GlyGly, GlySer, SerGly, LeuGly, GlyLeu, SerMet, AlaSer, LeuSer, ValSer, GlyThr, AlaThr, GlyGlyGly) were measured at pH 7-10, 70℃. Hydrolytic cleavage of pBR322 DNA with Cu(II)TACH-X(X=H, Me, Et, 2Et, 3Et) were investigated at pH 7-9.5, 35℃ to study the reaction mechanism and the effect of X. Cyclic DNA(pHSG398, pUC19, pBR322, φX174, ColE1) were also cleaved with Cu(II)TACH to investigate the DNA size effect. Also, a new method to determine the scission site of DNA was established.