METAL COMPLEX CATALYZED HYDROLYSIS OF PHOSPHATE ESTER

金属配合物催化磷酸酯水解

• 批准号: 6271490
• 负责人: YILMA GULTNEH
• 金额: $ 6.35万
• 依托单位: HOWARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6271490
• 关键词: 2,4 dinitrophenol; X ray crystallography; acid base balance; active sites; catalyst; chemical structure function; chemical synthesis; copper; enzyme activity; hydrolysis; infrared spectrometry; ligands; metal complex; nickel; phosphoric ester; zinc

The long term objectives and specific aims of this project are twofold; a) to further our ongoing research project in the synthesis and detailed studies of mono-and multi-nuclear transition metal complexes in an attempt to mimic aspects of the active sites of the hydrolytic enzymes and contribute toward the effort to understand the mechanisms of enzymatic catalysis, and; b) equally importantly, to recruit, involve, and train minority graduate and undergraduate students in the medical/biologically related chemical research as part of their training as prospective professionals in the biomedical field of research. The hydrolytic metallo-enzymes play crucial roles in the metabolism of all living organisms and hence there is a need to understand their mechanism of general and specific but always highly efficient, catalysis of vital reactions such as the hydrolysis of biomolecules such as DNA, RNA, carbohydrates, fats, and proteins. It is proposed to synthesize a range of rationally designed and progressively modified ligands and their Zn(II), Ni(II), and Cu(II) complexes, purify and study in detail their X-ray crystallographic structure, spectroscopic properties, and their acid-based and metal complex stability equilibria in Aqueous solutions under various conditions of solvents and pH. Detailed studies will be carried out of the catalytic properties these complexes initially for the hydrolysis of small molecules with biologically relevant functional groups such as phosphate and carboxylic acid esters and peptide (amide) bonds and at later stages actual biological molecules. Catalytic reaction products will be identified, the kinetics of the reactions studied, and investigations made into the relationship of the ligand and metal complex characteristics to the catalytic properties and how these findings help in understanding the enzymes. Student Participation and training in scientific research: Our equally emphasized focus and aim for this project is the training of minority undergraduate and graduate students in the techniques and methodology in the chemical research field related to chemical/biological and medical fields. We aim to recruit promising graduate and undergraduate minority students within howard university and from other schools and colleges at the early stages of their studies to involve students in our ongoing research work with active guided participation of students at every stage of the work from literature studies, design of experiments, executing experiments, interpreting data and communicating research finding in seminars and in publications. That has been the track record of the research group and it will be continued with the financial support obtained.

该项目的长期目标和具体目标是双重的；一个） 进一步推进我们正在进行的研究项目的综合和详细研究 单核和多核过渡金属配合物的尝试研究 模仿水解酶活性位点的各个方面， 有助于理解酶促机制 催化，以及； b) 同样重要的是，招募、参与和培训 医学/生物学领域的少数民族研究生和本科生 相关化学研究作为他们前瞻性培训的一部分 生物医学研究领域的专业人士。 水解金属酶在所有物质的代谢中起着至关重要的作用 生物体，因此需要了解它们的机制 一般和具体但总是高效的，催化重要的 DNA、RNA 等生物分子的水解等反应 碳水化合物、脂肪和蛋白质。建议综合一系列 合理设计和逐步修饰的配体及其Zn(II)， Ni(II) 和 Cu(II) 配合物，纯化并详细研究其 X 射线 晶体结构、光谱性质及其酸基 不同条件下水溶液中金属配合物的稳定性平衡 溶剂条件和pH值。将进行详细研究 这些配合物的催化特性最初用于小分子的水解 具有生物学相关官能团的分子，例如磷酸盐 以及羧酸酯和肽（酰胺）键，并在后期 实际的生物分子。催化反应产物将是 确定、研究反应动力学并进行调查 深入研究配体与金属配合物特性的关系 催化特性以及这些发现如何帮助理解 酶。 学生参与科学研究和培训：我们的平等 该项目强调的重点和目标是少数民族的培训 本科生和研究生的技术和方法 与化学/生物和医学相关的化学研究领域 字段。我们的目标是招募少数有前途的研究生和本科生 霍华德大学以及来自其他学校和学院的学生 在他们学习的早期阶段让学生参与我们正在进行的 每个阶段学生积极引导参与的研究工作 从文献研究、实验设计、执行 实验、解释数据和交流研究发现 研讨会和出版物。这是该公司的业绩记录 研究小组将在财政支持下继续进行 获得。