Development of a device for analyzing fine particles by exploiting acoustic radiation force and electrostatic force

作为开发分析生物细胞中各种成分的装置的第一步，根据先前的研究组装了通过声辐射力浓缩液体中纳米颗粒的装置，并通过视频显微镜观察颗粒分布。几个变量，如颗粒浓度，液体中的颗粒的密度匹配，颗粒直径，通道的内壁涂层，和超声波的峰-峰振幅进行了检查。不幸的是，该装置以不可再现的方式工作，并且没有获得可靠的结果。除此之外，通道的化学状态在这种装置的设计中起着重要的作用，因为它可以影响表面上产生的电渗的大小以及分离能力。二氧化硅表面涂有二氧化钛晶体形式的二氧化硅。所得表面具有阳极或阴极电渗，这取决于溶液的pH和组成。二氧化钛表面容易改性硅烷化试剂，使一些色谱相互作用发生。

As the first step toward developing a device by which various constituents in a biological cell are analyzed, a device for concentrating nanoparticles in liquid by acoustic radiation force was assembled according previous studies, and particle distribution was observed by video microscopy. Several variables such as particle concentration, density matching of particles in liquid, particle diameter, inner wall coating of channels, and peak-to-peak amplitude of ultrasound were examined. Unfortunately, the device works in an irreproducible manner and reliable results were not obtained. Apart from this, the chemical state of the channels plays an important part in the design of such a device, because it can affect the magnitude of electroosmosis generated on the surface as well as the separation ability.Silica surface was coated with titanium dioxide in a crystalline form of anatase. The resultant surface had anodic or cathodic electroosmosis, depending on the pH and composition of the solution. Titanium dioxide surface was readily modified by a silanizing reagent so that some chromatographic interaction took place.