Studies in the distribution of yieldin in cowpea seedlings

我们用一种抗YIELDIN抗体检测了YEYELDIN在豌豆黄化幼苗中的分布和免疫组织化学定位。免疫印迹分析表明，Yaieldin位于气生器官(胚芽、上胚轴和下胚轴)中，而不在根中。下胚轴在顶端伸长前区域(钩区)的Yaieldin信号强度最高，向伸长的成熟基部方向减弱，表明随着细胞伸长的停止，Yaieldin信号消失。利用不同萌发阶段的下胚轴进行的组织印迹免疫印迹分析支持这一观点，因为顶端富含Yayeldin的区域位于子叶节正下方(钩部和快速伸长区域)，与下胚轴的伸长一起顶端迁移。免疫组织化学显微镜显示Yayeldin定位于胚轴的皮质和表皮的细胞壁。这一结果与Yaieldin参与调节伸长生长过程中细胞壁屈服的观点是一致的。

We examined the distribution and the immunohistochemical localization of yieldin in etiolated cowpea seedlings with an anti-yieldin antibody. An immunoblotting analysis revealed that the yieldin was located in the aerial organs (plumule, epicotyl and hypocotyl) but not in the roots. The intensity of the yieldin signal in the hypocotyls was highest in the apical pre-elongation region (the hook region) and decreased toward the elongated mature base indicating that the yieldin disappeared with the ceasing of cell elongation. Tissue-print immunoblotting analysis using hypocotyls in different germination stages supports this view because the apical yieldin-rich regions, just beneath the cotyledonary node (the hook and rapidly elongating regions), acropetally migrated together with hypocotyl elongation.Immunohistochemical microscopy demonstrated that yieldin was localized in the cell walls of the cortex and epidermis of the germ axes. The present results are consistent with the view that yieldin participates in the regulation of cell wall yielding during elongation growth.