Development of a novel disposable film culture system using a high gas-permeable, thermal-stable and multilayer film

本研究的目的是：(1)研究一种与氟碳聚合物膜（PFA）性质相似的超廉价薄膜OTP膜（OTP）是否满足“Culture Pack”-Rockwool系统（CP-RW）的要求；(2)开发一种新的OTP膜培养容器，即“Vitron”，它可以很好地用于各种植物的体外克隆苗的培养；并希望“Vitron”将广泛应用于商业组织培养实验室。本研究使用的外植体是从3个蕙兰品种(1)的大量离体芽中获得的三片叶片的芽，以及尤加利、甘薯、草莓和大量蕙兰。(2)原球茎样体（PLBs）。底物为RW multiblock或Oasis加无糖液体培养基，含糖琼脂加液体培养基。将膜培养系统置于25℃高CO_2富集（3000 ppm）条件下的培养室中。在CP-RW中，使用OTP对大花蕙兰的茎和根生长均有促进作用，其效果与PFA相当。在两种膜培养系统中，植株的生长都比在常规系统（用琼脂培养基的烧瓶）中得到了更多的促进。使用OTP和PFA在CP-RW中培养的植株光合速率高于烧瓶培养的植株。结果表明，OTP是一种良好的培养容器材料。与使用OTP和PFA的“奇迹包”（CP的实用模型）相比，“Vitron”处理的桉树和甘薯苗的生长明显加快。在“Vitron”中草莓的生长和PLB的增殖与OTP和PFA体系相当。这些结果表明，“Vitron”作为一种模式培养容器具有很大的潜力，因为该系统可以为各种植物物种的体外生长提供良好的环境。此外，该容器易于一次性使用，从而减少了洗涤的人工成本。通过应用这种容器系统，许多重要的园艺、农业和林业植物的工业生产将可以进行微繁繁殖。少

The purposes of this study are (1) to investigate whether or not a novel OTP film (OTP), a super cheap film with similar characters as fluorocarbon polymer film (PFA), meets the requirements for the "Culture Pack"-Rockwool system (CP-RW), and (2) then to develop a new film culture vessel made of OTP, namely the "Vitron" that can be well-suited for the development of in vitro clonal plantlets of various plant species; and in a hope that the "Vitron" will be used widely in commercial tissue culture laboratories. The explants used in this study were shoots with three leaves obtained from a mass of in vitro shoots of three Cymbidium cultivars1 for exp. (1), and Eucalyptus uro-grandis, sweet potato, strawberry and a mass of Cym.protocorm-like bodies (PLBs) for exp. (2). The substrates used were RW multiblock or Oasis with sugar-free liquid medium, and sugar-containing agar and liquid medium. The film culture systems were placed in the culture room at 25℃ under high CO_2 enriched (3000 ppm) … More or non-enriched condition. Enhanced shoot and root growth of Cymbidium were observed in plantlets grown in CP-RW using OTP and the value was comparable to that of PFA. The growth of plantlets were enhanced more in both film culture systems than in a conventional system (flask with agar medium). Photosynthetic rate of the plantlets cultured in CP-RW using OTP and PFA was higher than that of the plantlets cultured in flask. These results indicate that OTP is a good material for developing culture vessel. The growth of Eucalyptus and sweet potato plantlets were drastically enhanced in the "Vitron" when compared with the "Miracle Pack" (the practical model of CP) using OTP and PFA. The growth of strawberry and the proliferation of PLB in the "Vitron" were comparable to that of OTP and PFA systems. These results shows great potential of the "Vitron" as a model culture vessel for micropropagation, since the system can facilitate a favorable environment for in vitro growth of various plantlet species. Moreover, the vessel is easily disposable thereby reducing labor cost for washing. By applying this vessel system, micropropagation would be accessible for industrial production of many important horticultural, agricultural and forestry plants. Less