Structure of modules of the respiratory NADH: Ubiquinone oxidoreductase (complex I)

质子泵NADH：泛醌氧化还原酶，又称呼吸复合体I，由于其组成复杂，人们对其结构和功能知之甚少。在细菌中制成的复合体的最小形式由亲水性NADH脱氢酶模块、两亲性连接模块和疏水膜模块构成。我们正试图从大肠杆菌和超嗜热菌Aquifex aeolicus中结晶该复合体及其模块。来自大肠杆菌的大肠杆菌复合体I的整体结构也将通过复合体I的二维膜和膜组件的电子冷冻显微镜来确定。

The structure and function of the proton-pumping NADH:ubiquinone oxidoreductase, also called respiratory complex I, is poorly understood due to its complex composition. A minimal form of the complex made in bacteria is constructed of a hydrophilic NADH dehydrogenase module, an amphipatic connecting module and a hydrophobic membrane module. We are trying to crystallize the complex and its modules from Escherichia coli and from the hyperthermophilic bacterium Aquifex aeolicus. The overall structure of the E. coli complex I from E coli will be also determined by electroncryomicroscopy of two-dimensional membranes of complex I and the membrane module.