REDUCTION OF IMMUNE MEMORY IN SPECIFIC IMMUNITY TO RHODOCOCCUS EQUI IN MICE MODEL

用活毒力R.马可保护小鼠免受有毒微生物的侵害，而热灭活的有毒或无毒的活细菌则不能提供保护。在亚致死接种活ATCC 33701后5天观察到保护性免疫，并且在攻击前9天致敏的小鼠中发现攻击细菌的最有效清除。免疫记忆由活的强毒R。几个星期后，我就回来了。本研究的目的是阐明还原反应的机理。小鼠模型中的等特异性免疫记忆。为检测免疫脾细胞产生IFN-γ和TNF-α的能力，用活的强毒R. Equi培养7、17、21和28天。免疫脾细胞经R.在免疫7天的小鼠中观察到马抗原，并且免疫17天的小鼠的脾细胞的产生水平变得与正常小鼠的相同。Sple ...更多信息 将免疫7天或14天的小鼠的en细胞转移到正常小鼠，并用强毒R.等。脾细胞致敏7天后转移免疫效果较好，14天后转移免疫效果不明显。用强毒力的R.用抗IL-10单克隆抗体免疫马，然后用强毒R.等。抗IL-10抗体对R.马的器官相比，那些未经处理的免疫小鼠。抗IL-10单克隆抗体的体外实验表明，IL-10对免疫脾细胞产生IFN-γ和TNF-α有明显的促进作用。使用抗CD 44、CD 62 L和CD 45 RB抗体，通过流式细胞术分析脾细胞上CD 44、CD 62 L和CD 45 RB抗原的表达。而免疫7天和21天的小鼠脾脏T细胞无明显变化。结论IL-10可能在红细胞免疫记忆的建立和减弱过程中起重要作用。马红细胞感染小鼠T细胞的灭活或耐受机制尚不清楚。马感染仍不清楚。少

Immunization with live virulent R. equi conferred protection against virulent organisms in mice, whereas heat-killed virulent or live avirulent bacteria offered no protection. The protective immunity was observed 5 days after the sublethal inoculation with live ATCC 33701, and the most effective clearance of challenged bacteria was found in the mice primed at 9 days before the challenge. Immune memory by the live virulent R. equi reduced for a few weeks. The purpose of this study is to elucidate the mechanism of the reduction of R. equi-specific immune memory in mice model. To measure the production of IFN-γ and TNF-α from immune spleen cells, mice were immunized with live virulent R. equi for 7, 17, 21, and 28 days. The highest production of IFN-γ and TNF- a from immune spleen cells after stimulation with R. equi antigens was observed in mice immunized for 7 days, and the production level of the spleen cell from mice immunized for 17 days became the same as that from normal mice. Sple … More en cells from mice immunized for 7 or 14 days were transfer to normal mice and the mice were challenged with virulent R. equi. The transfer immunity was effective in mice received spleen cell primed for 7 days, but not for 14 days. Immunized mice for 7 or 21 days with virulent R. equi were inoculated with anti-IL10 monoclonal antibody, and then challenged with virulent R. equi. There was no effect of anti-IL-10 antibody on the clearance of R. equi in the organs as compared with those of none-treated immune mice. On the other hand, in vitro experiment with anti-IL-10 monoclonal antibody showed effective function of IL-10 on the production of IFN-γ and TNF-α from immune spleen cells. Expression of CD44, CD62L, and CD45RB antigens on the spleen cells were analyzed by flow cytometory analysis using anti-CD44, CD62L, and CD45RB antibodies. However, there was no change between spleen T cells from immunized mice for 7 and 21 days. In conclusion, IL-10 might play a key role in the establishment and reduction of immune memory in R. equi infection in mice, however, the mechanism of inactivation or tolerance of T cells by R. equi infection remain unclear. Less