Cancer immunotherapy using dendritic cells and chimeric monoclonal antibody which induce apoptosis

树突状细胞是最重要的抗原呈递细胞。最近的许多研究比较了未成熟和成熟树突状细胞的功能，但关于成熟过程中树突状细胞发生的分子变化的报道很少。本研究报告了外周血单核细胞生成的未成熟dc与成熟dc的基因表达差异。用低浓度脂多糖激活未成熟树突状细胞诱导成熟后，采用差异展示法评估基因表达。蛋白酶体亚基（PSMA3）、TFEC异构体和BTK区克隆2f10-rpi瞬间上调。色氨酸- trna合成酶和CD63抗原上调至少24小时。神经元凋亡抑制蛋白（NAIP）和转化生长因子β诱导的68 kD蛋白下调。这是首次报道NAIP在人DCs中的表达，将NAIP的表达与凋亡抑制蛋白（IAP）家族和bcl-2家族的其他成员的表达进行比较，只有NAIP在LPS刺激前的未成熟DCs中强烈表达。免疫复合物刺激的dc也能诱导PSMA3。这些发现可能有助于我们理解DC成熟和DC基疫苗的有效性。

Dendritic cells (DCs), are the most important antigen presenting cells. Many recent studies have compared the function of immature and mature DCs, but there have been few reports of the molecular changes that occur in DCs during maturation. Here we report differential gene expression in immature DCs generated from peripheral blood monocytes compared with mature DCs. Gene expression was evaluated using the differential display method after activation of immature DCs with a low concentration of lipopolysaccaride to induce maturation. Proteasome subunit (PSMA3), TFEC isoform, and BTK region clone 2f10-rpi were transiently up-regulated. Tryptophanyl-tRNA synthetase and CD63 antigen were up-regulated for at least 24 hours. Neuronal apoptosis inhibitory protein (NAIP) and transforming growth factor beta-induced 68 kD protein were down-regulated. This is the first report of NAIP expression in human DCs, Comparing the expression of NAIP with that of other members of the inhibitor of apoptosis protein (IAP) family and the bcl-2 family, only NAIP was strongly expressed in immature DCs before stimulation by LPS. PSMA3 was also induced in DCs stimulated with immune complex. These findings might contribute to our understanding of DC maturation and the effectiveness of DC-based vaccines.