The study on the cross-talk between vitamin D and TGFβ signal transduction paihways in the pancreatic cancer cell lines

1.本研究的目的是阐明维生素D和转化生长因子β信号转导通路之间的相互作用是否在胰腺癌细胞系中起作用。同时对维生素D和转化生长因子β反应良好的胰腺癌细胞株SUP-1不表达作用于转化生长因子β信号转导通路末端的Smad4，提示维生素D和转化生长因子β信号转导通路之间可能存在相互作用。结论：1.经转化生长因子β处理后，维生素D受体在VdR反应元件转导的Die SuP-1细胞中未观察到拮抗活性，因此不能确定维生素D与转化生长因子β信号转导通路之间的相互作用。本研究旨在探讨PPARg配体曲格列酮是否具有抑制胰腺癌细胞生长的作用。曲格列酮通过上调p21的表达，对胰腺癌细胞株具有生长抑制和诱导分化作用，为胰腺癌的有效治疗提供新的分子靶点。自身免疫性胰腺炎是一种以血清IgG4浓度升高为特征的疾病，我们研究了人类白细胞抗原等位基因与自身免疫性胰腺炎的关系。DRB1*0405-DQB1*0401单倍型与自身免疫性胰腺炎显著相关，可能在抗原提呈和诱导自身免疫反应中发挥作用。

1. The aim of die present study is to clarify whether the cross-talk between vitamin D and TGFβ signal Iransduclion pathways is operating in the pancreatic cancer cell lines. Pancreatic cancer cell line SUP-1, which well responds to both vitamin D and TGFβ, shows no expression of smad4 which acts at the terminal portion of TGFβ signal transduction pathways, suggesting that the cross-talk between vitamin D and TGFβ signal transduction pathways may be operating. However, no irancriptive activity of vitamin D receptor was observed in Die SUP-1 transfectecl by VDR response element after TGFβ treatment Accordingly, we cannot confirm he cross-talk between vitamin D and TGFβ signal transduction pathways.2. The aim of this study is to assess whether the PPARg ligand, troglilazone, inhibits' the growth of pancreatic cancer cells., Troglilazone, had growth inhibitory, and differentiation induction effects on die pancreatic ameer cell lines.through the up-expression of p21, suggesting a new molecular target for effective therapy against pancreatic cancer.3. Autoimmune pancreatitis is a distinctive disease entity characterized by high serum IgG4 concentrations, and we investigated the association between HLA alleles and autoimmune pancreatitis. The DRB1*0405-DQB1*0401 haplolype is significantly associated with autoimmune pancreatitis and may play a functional role in antigen presentation and the induction of an autoimmune response.