Role of gastric leptin and cholecystokinin in patients with functional dyspepsia

本研究以功能性消化不良患者和健康志愿者为研究对象。他们都对胃镜下采血和胃粘膜活检给予了书面知情同意。本研究得到了我所伦理委员会的批准。用放射免疫法测定血浆CCK和瘦素水平。在内窥镜检查期间对眼底部位进行活组织检查。用兔抗人瘦素多克隆抗体(A-20，Santa Cruz Biotech，美国加利福尼亚州)进行瘦素免疫染色。功能性消化不良患者与健康志愿者的血浆CCK和瘦素水平均无显著差异。瘦素免疫反应细胞见于兵团上皮腺的下半部。上皮细胞中瘦素阳性染色的强度以5分为标准。功能性消化不良患者与健康志愿者瘦素阳性染色强度无明显差异。此外，幽门螺杆菌阴性组和阳性组之间、体重指数高水平(≧23)和低水平(Lt；23)之间、老年(≧70)和非老年(&lt；70)组之间瘦素的阳性染色强度没有显著差异。需要进一步检测胃粘膜瘦素和十二指肠粘膜CCK mRNA。

The patients with functional dyspepsia and healthy volunteers were investigated in the study. They all gave written informed consent for blood sampling and gastric mucosal biopsy by endoscopy. This study was approved by the Ethics Committee of our institute. Plasma CCK and leptin were estimated using a RIA kit. Biopsies of fundic sites were performed during endoscopic examination. Immunostaining for leptin was performed by a polyclonal rabbit antihuman leptin antibody (A-20, Santa Cruz Biotechnology, California, USA). Neither plasma CCK nor leptin levels differed significantly between the patients with functional dyspepsia and healthy volunteers. Leptin immunoreactive cells were found in the lower half of the epithelial glands of the corps. Intensity of positive staining for leptin in the epithelial cells was rated on a 5-point score. There was no significant difference in intensity of positive staining for leptin between the patients with functional dyspepsia and healthy volunteers. In addition, there was no significant difference in intensity of positive staining for leptin between the H. pylori-negative and -positive groups, between the high (≧23) and low (<23) levels of body mass index groups or between the aged (≧70) and not aged (<70) groups. Further determination of gastric mucosal leptin mRNA and duodenal mucosal CCK mRNA was required.