Development and verification of new agent ^<99m>Tc-technenano-colloid for lymphoscintigraphy

恶性黑色素瘤或乳腺癌的前哨淋巴结概念吸引了放射性核素显像。我厂没有最佳粒径的淋巴造影剂。我们试图研制出最佳粒径的新制剂--<99m>锝-纳米胶体。我们制作了一种新的收集~ 1 <99m>Tc-Technegas到盐水溶液中的设备，并将其命名为Jikei University Style的撞击器。我们对微粒的大小、均匀性、结合情况进行了验证，并尝试用~（13）<99m>Tc-锝胶体对家兔进行淋巴管造影，观察淋巴管的形成及淋巴结内的积聚情况。“乳腺癌患者的淋巴结定位和前哨淋巴结活检以确定腋窝淋巴结清扫术”我们与外科合作，进行了腋窝淋巴结定位和前哨淋巴结造影，第一，是否单个腋窝淋巴结（前哨淋巴结）最初接收来自乳腺癌的恶性细胞，其次明确的前哨淋巴结是否可靠地预测疾病-在连续的乳腺癌手术系列中，我们<99m>在手术前一天在肿瘤部位附近皮下注射13 Tc-植酸盐，并拍摄腋窝和乳房的X线摄影图像。术中对前哨淋巴结进行病理学检查。不存在定义为前哨淋巴结检测为阴性而较高淋巴结检测为阳性的“跳跃”转移。为了获得更好的图像，提高前哨淋巴结的检出率，我们研制了一种新型前哨淋巴结造影屏蔽体。这种新的防护罩可以很容易地覆盖注射部位，以防止晒伤。我们开发了自己的考试方法;-同时标记法。

Lymphoscintigraphy has been attracted for the reason of sentinel node concept for malignant melanoma or breast cancer. We don't have the agent with best particle size for lymphoscintigraphy in this countory. We have tried to develop new agent, ^<99m>Tc-technenano-colloid with best size of particle. We made the new equipment which collected ^<99m>Tc-technegas into saline solution and named it the impinger of Jikei University Style. We verified the size, uniformity, bonding of the particle.We tried to perform lymphoscmtigraphy with ^<99m>Tc-technenano-colloid to rabbits and observed the lymphatic basin and accumulation in lymph nodes. "Lymphatic mapping and sentinel node biopsy in patient with breast cancer for determination axillary lymph node disection" In cooperation with Department of Surgery, we have performed lympatic mapping and sentinel node scintigraphy to assess, first whether a single axillary node (sentinel node) initially receives malignant cells from a breast carcinoma and second whether a clear sentinel node reliably forecasts a disease-free axilla.In consecutive series with operative breast carcinoma, we injected ^<99m>Tc-phytate subdermally close to the tumor site, on the day before surgery, and scintigraphic images of the axilla and breast were taken. The sentinel node was performed intraoperative pathological examination. There were no "skip" metastases defined as a sentinel node that tested negative with higher nodes that tested positive. We have developed the new shield for sentinel lymph node scintigraphy to get better image and improve detectability of sentinel lymph node. This new shield is covered the injection site easily to prevent shine thrbugh. We developed our own examination method;-Simultaneous marking method.